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Immunology and Infection

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JoVE Journal Immunology and Infection
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Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining

Article DOI: 10.3791/58978-v • 08:04 min • February 8th, 2019
February 8th, 2019 •
1, 2, 2, 2, 2, 2
1Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, 2Department of Cell Biology and Molecular Genetics, University of Maryland

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Summary
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A simple ATP-measuring assay and live/dead staining method were used to quantify and visualize Neisseria gonorrhoeae survival after treatment with ceftriaxone. This protocol can be extended to examine the antimicrobial effects of any antibiotic and can be used to define the minimal inhibitory concentration of antibiotics in bacterial biofilms.

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Quantitative Examination Antibiotic Susceptibility Neisseria Gonorrhoeae Aggregates ATP-utilization Commercial Assays Live/dead Staining Antibiotic Concentration Bacteria Aggregates Antimicrobial Susceptibility Lysis Steps ATP Measuring Assay Viable Bacteria Visualization Method Neisseria Gonorrhoeae Strains GCK Agar Pili-negative Colonies Pili-positive Colonies GCK Plates Sterile Applicator Warm Broth Sodium Bicarbonate
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