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Journal / Bioengineering / Designing Porous Silicon Films as Carriers of…
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Bioengineering

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JoVE Journal Bioengineering
Designing Porous Silicon Films as Carriers of Nerve Growth Factor
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Designing Porous Silicon Films as Carriers of Nerve Growth Factor

Article DOI: 10.3791/58982-v • 10:12 min • January 25th, 2019
January 25th, 2019 •
1, 2,3, 2,3, 1,4
1Department of Biotechnology and Food Engineering, Technion - Israel Institute of Technology, 2Faculty of Engineering, Bar-Ilan University, 3Bar-Ilan Institute of Nanotechnologies and Advanced Materials, 4Russell Berrie Nanotechnology Institute, Technion - Israel Institute of Technology

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Summary
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Here, we present a protocol to design and fabricate nanostructured porous silicon (PSi) films as degradable carriers for the nerve growth factor (NGF). Neuronal differentiation and outgrowth of PC12 cells and mice dorsal root ganglion (DRG) neurons are characterized upon treatment with the NGF-loaded PSi carriers.

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Porous Silicon Films Nerve Growth Factor (NGF) Carrier Release Bioactive Protein Loading Room Temperature Organic Solvents Tunable Design Reservoir Implants Factors And Drugs Fabrication Process Cell Culture Procedures Silicon Wafer Resistivity Aluminum Foil O-ring Electrochemical Etching Hydrofluoric Acid Ethanol Nitrogen Stream Thermal Oxidation
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