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JoVE Journal Biochemistry
Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
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Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Article DOI: 10.3791/58996-v • 07:04 min • February 5th, 2019
February 5th, 2019 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, University of Southern Denmark

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Site-directed mutagenesis is a technique used to introduce specific mutations in deoxyribonucleic acid (DNA). This protocol describes how to do site-directed mutagenesis with a 2-step and 3-step polymerase chain reaction (PCR) based approach, which is applicable to any DNA fragment of interest.

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Site-directed Mutagenesis In Vitro Experiments In Vivo Experiments RNA Interactions Escherichia Coli PCR Approach DNA Template Primer Pairs Agarose Gel Electrophoresis Ethidium Bromide Gel Extraction Kit Spectrophotometer
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