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JoVE Journal Bioengineering
Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
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Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Article DOI: 10.3791/59123-v • 07:39 min • January 22nd, 2019
January 22nd, 2019 •
1,2,3, 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

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Summary
Automatic Translation

Here, a protocol is presented for encapsulating and culturing cells in poly(ethylene glycol) (PEG) hydrogels functionalized with a fluorogenic matrix metalloproteinase (MMP)-degradable peptide. Cellular MMP and metabolic activity are measured directly from the hydrogel cultures using a standard microplate reader.

Tags

Global Cellular Matrix Metalloproteinase Metabolic Activity 3D Hydrogels Measurement Standard Biological Assays Fluorescent Sensor Microplate Reader High Throughput Drug Screening Proteases Cell Functions Experiment Planning Hydrogel Preparation Assay Media Charcoal Stripped Fetal Bovine Serum L-Glutamine Penicillin Streptomycin Phenol Red Media Positive Controls Bacterial Collagenase Enzyme Type One Hydrogel Precursor Solution 1.5 ML Tube Cell Encapsulation
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