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Journal / Genetics / Stabil knockdown af gener kodning Ekstracellulære …
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Genetics

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JoVE Journal Genetics
Stabil knockdown af gener kodning Ekstracellulære Matrix Proteiner i C2C12 Myoblast Cell Line Ved hjælp af Small-Hairpin (sh)RNA
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Stabil knockdown af gener kodning Ekstracellulære Matrix Proteiner i C2C12 Myoblast Cell Line Ved hjælp af Small-Hairpin (sh)RNA

Article DOI: 10.3791/60824-v • 12:19 min • February 12th, 2020
February 12th, 2020 •
1, 1, 1
1Orthopaedic Research Laboratories, Leni & Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai

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Vi leverer en protokol til stably knockly nedslag gener kodning ekstracellulære matrix (ECM) proteiner i C2C12 myoblaster ved hjælp af små hårnål (sh) RNA. Målretning ADAMTSL2 som et eksempel, beskriver vi metoderne til validering af knockdown effektivitet på mRNA, protein, og cellulære niveau under C2C12 myoblast til myotube differentiering.

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Genetik ADAMTS proteases ADAMTS-lignende proteiner C2C12 myoblaster ekstracellulære matrix skeletmuskulatur differentiering myogenese shRNA
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