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Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model
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Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model

Article DOI: 10.3791/61008-v • 12:23 min • April 24th, 2020
April 24th, 2020 •
1,2, 1,3, 1, 1, 1, 1
1Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital, University of Sydney, 2HTRG - Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery, Heidelberg University Hospital, 3Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University

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Summary
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Here, we describe a detailed and reproducible flow cytometry protocol to identify monocyte/macrophage and T-cell subsets using both extra- and intracellular staining assays within the murine spleen, bone marrow, lymph nodes and synovial tissue, utilizing an established surgical model of murine osteoarthritis.

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Flow Cytometry Immune Cell Subsets Spleen Bone Marrow Lymph Nodes Synovial Tissue Osteoarthritis Model Monocytes Macrophages T-cells Neurons Ovium Immune System Harvest Immune Cells Synovium Osteoarthritic Immune Response Isolation Of Tissues Mouse Model Supine Position 70% Ethanol Scissors Skin Opening Adipose Tissue Dissecting Microscope Forceps Inguinal Lymph Node
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