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Journal / Cancer Research / 单核RNA-seq和ATAC-seq的新鲜冷冻脑肿瘤的核隔离…
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JoVE Journal Cancer Research
单核RNA-seq和ATAC-seq的新鲜冷冻脑肿瘤的核隔离
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单核RNA-seq和ATAC-seq的新鲜冷冻脑肿瘤的核隔离

Article DOI: 10.3791/61542-v • 06:22 min • August 25th, 2020
August 25th, 2020 •
1, 2, 1, 1,3, 4, 2, 1
1Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, 2Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), 3Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ), 4Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, INF 400, Heidelberg, Germany

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肿瘤内异质性是肿瘤的内在特征，包括胶质瘤。我们开发了一个简单而高效的协议，利用缓冲和梯度离心的组合，将单核从新鲜冷冻胶质瘤组织中分离出来，用于单核RNA和ATAC测序研究。

Tags

癌症研究， 第 162 期， 核隔离， snrna - seq， snatac - seq， 10 倍基因组学， 胶质瘤
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