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JoVE Journal Cancer Research
Nuclei Isolation fra friske frosne hjernetumorer til Single-Nucleus RNA-seq og ATAC-seq
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Nuclei Isolation fra friske frosne hjernetumorer til Single-Nucleus RNA-seq og ATAC-seq

Article DOI: 10.3791/61542-v • 06:22 min • August 25th, 2020
August 25th, 2020 •
1, 2, 1, 1,3, 4, 2, 1
1Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, 2Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), 3Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ), 4Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, INF 400, Heidelberg, Germany

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Intra-tumoral heterogenitet er et iboende træk ved tumorer, herunder gliomer. Vi udviklede en enkel og effektiv protokol, der bruger en kombination af buffere og gradient centrifugering til at isolere enkelt kerner fra friske frosne gliomvæv til enkelt kerne RNA og ATAC sekventering undersøgelser.

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Kræftforskning Udgave 162 Nuclei isolation snRNA-seq snATAC-seq 10x Genomforskning Glioma
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