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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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JoVE Applied Physics
JoVE Applied Physics encompasses a broad range of experimental and instrumental techniques utilized in physics research. Investigations in this area strive to address and answer a broad range of scientific questions, such as device mechanisms and efficiencies, using physical tools. This approach often requires a combination of specialties, and research in this area tends to be interdisciplinary with contributions from mechanical, electrical, and chemical engineers.
 JoVE Applied Physics

Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes

1Jülich Supercomputing Centre, Forschungszentrum Jülich, 2Materials Science and Technology of Polymer, MESA+ Institute for Nanotechnology, University of Twente, 3Department of Materials Science and Engineering, Universität des Saarlandes


JoVE 52285

The methodology to perform friction force microscopy experiments for contacting brushes is presented: Two polymer brushes that are grafted from (a) substrates and (b) colloidal probes are slid to show that, by using two contacting immiscible brush systems, friction in sliding contacts is reduced compared to miscible brush systems.

 JoVE Applied Physics

Novel 3D/VR Interactive Environment for MD Simulations, Visualization and Analysis

1Materials Science and Engineering, School of Engineering, University of California Merced


JoVE 51384

A new computational system featuring GPU-accelerated molecular dynamics simulation and 3D/VR visualization, analysis and manipulation of nanostructures has been implemented, representing a novel approach to advance materials research and promote innovative investigation and alternative methods to learn about material structures with dimensions invisible to the human eye.

 JoVE Applied Physics

A Testing Platform for Durability Studies of Polymers and Fiber-reinforced Polymer Composites under Concurrent Hygrothermo-mechanical Stimuli

1Department of Mechanical and Aerospace Engineering, University of California, Davis


JoVE 52464

The durability of polymers and fiber-reinforced polymer composites in service is a critical aspect for their designs and condition-based maintenance. We present a novel low-cost laboratory testing platform for the investigation of the influence of concurrent mechanical and environmental loadings, and may help design more efficient yet safer composite structures.

 JoVE Applied Physics

Patterning via Optical Saturable Transitions - Fabrication and Characterization

1Department of Electrical and Computer Engineering, The University of Utah, 2Department of Chemistry, The University of Wisconsin-Madison


JoVE 52449

We report that the diffraction limit of conventional optical lithography can be overcome by exploiting the transitions of organic photochromic derivatives induced by their photoisomerization at low light intensities.1-3 This paper outlines our fabrication technique and two locking mechanisms, namely: dissolution of one photoisomer and electrochemical oxidation.

 JoVE Applied Physics

Casting Protocols for the Production of Open Cell Aluminum Foams by the Replication Technique and the Effect on Porosity

1Department of Materials Science and Engineering, The University of Sheffield, 2Department of Mechanical Engineering, The University of Sheffield


JoVE 52268

Replication is one of the processing techniques used for the production of porous metal sponges. In this paper one implementation of the method for the production of open celled porous aluminum is shown in detail.

 JoVE Applied Physics

Convergent Polishing: A Simple, Rapid, Full Aperture Polishing Process of High Quality Optical Flats & Spheres

1Lasers, Optics, & Targets for the National Ignition Facility, Lawrence Livermore National Laboratory


JoVE 51965

A novel optical polishing process, called “Convergent Polishing”, which enables faster, lower cost polishing, is described. Unlike conventional polishing processes, Convergent Polishing allows a glass workpiece to be polished in a single iteration and with high surface quality to its final surface figure without requiring changes to polishing parameters.

 JoVE Applied Physics

Mechanical Expansion of Steel Tubing as a Solution to Leaky Wellbores

1Craft and Hawkins Department of Petroleum Engineering, Louisiana State University


JoVE 52098

This article reports on a laboratory scale investigation of an existing field procedure and its adaptation for sealing of leaky wellbores. It consists of mechanical expansion of metal pipe, which results in an improved metal/cement bond, ultimate sealing of hydraulic pathways and prevention of gas leaks caused by the presence of a microannular channel.

 JoVE Applied Physics

Scalable Nanohelices for Predictive Studies and Enhanced 3D Visualization

1Materials Science and Engineering, School of Engineering, University of California Merced, 2Computer Science and Engineering, School of Engineering, University of California Merced


JoVE 51372

Accurate modeling of nanohelical structures is important for predictive simulation studies leading to novel nanotechnology applications.  Currently, software packages and codes are limited in creating atomistic helical models.  We present two procedures designed to create atomistic nanohelical models for simulations, and a graphical interface to enhance research through visualization.

 JoVE Applied Physics

In Situ Neutron Powder Diffraction Using Custom-made Lithium-ion Batteries

1School of Chemistry, University of Sydney, 2Institute for Superconducting & Electronic Materials, University of Wollongong, 3Australian Synchrotron, 4Australian Nuclear Science and Technology Organisation, 5School of Mechanical, Materials, and Mechatronic Engineering, University of Wollongong, 6School of Chemistry, University of New South Wales


JoVE 52284

We describe the design and construction of an electrochemical cell for the examination of electrode materials using in situ neutron powder diffraction (NPD). We briefly comment on alternate in situ NPD cell designs and discuss methods for the analysis of the corresponding in situ NPD data produced using this cell.

 JoVE Applied Physics

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

1Department of Mathematics, Physics and Statistics, University of the Sciences


JoVE 52091

The protocol for fabrication and operation of field dewetting devices (Field-DW) is described, as well as the preliminary studies of the effects of electric fields on droplet contents.

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