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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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Essentials of
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JoVE Applied Physics
JoVE Applied Physics encompasses a broad range of experimental and instrumental techniques utilized in physics research. Investigations in this area strive to address and answer a broad range of scientific questions, such as device mechanisms and efficiencies, using physical tools. This approach often requires a combination of specialties, and research in this area tends to be interdisciplinary with contributions from mechanical, electrical, and chemical engineers.
 JoVE Applied Physics

Mechanical Expansion of Steel Tubing as a Solution to Leaky Wellbores

1Craft and Hawkins Department of Petroleum Engineering, Louisiana State University


JoVE 52098

This article reports on a laboratory scale investigation of an existing field procedure and its adaptation for sealing of leaky wellbores. It consists of mechanical expansion of metal pipe, which results in an improved metal/cement bond, ultimate sealing of hydraulic pathways and prevention of gas leaks caused by the presence of a microannular channel.

 JoVE Applied Physics

Scalable Nanohelices for Predictive Studies and Enhanced 3D Visualization

1Materials Science and Engineering, School of Engineering, University of California Merced, 2Computer Science and Engineering, School of Engineering, University of California Merced


JoVE 51372

Accurate modeling of nanohelical structures is important for predictive simulation studies leading to novel nanotechnology applications.  Currently, software packages and codes are limited in creating atomistic helical models.  We present two procedures designed to create atomistic nanohelical models for simulations, and a graphical interface to enhance research through visualization.

 JoVE Applied Physics

In Situ Neutron Powder Diffraction Using Custom-made Lithium-ion Batteries

1School of Chemistry, University of Sydney, 2Institute for Superconducting & Electronic Materials, University of Wollongong, 3Australian Synchrotron, 4Australian Nuclear Science and Technology Organisation, 5School of Mechanical, Materials, and Mechatronic Engineering, University of Wollongong, 6School of Chemistry, University of New South Wales


JoVE 52284

We describe the design and construction of an electrochemical cell for the examination of electrode materials using in situ neutron powder diffraction (NPD). We briefly comment on alternate in situ NPD cell designs and discuss methods for the analysis of the corresponding in situ NPD data produced using this cell.

 JoVE Applied Physics

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

1Department of Mathematics, Physics and Statistics, University of the Sciences


JoVE 52091

The protocol for fabrication and operation of field dewetting devices (Field-DW) is described, as well as the preliminary studies of the effects of electric fields on droplet contents.

 JoVE Applied Physics

Ambient Method for the Production of an Ionically Gated Carbon Nanotube Common Cathode in Tandem Organic Solar Cells

1Physics Department, The University of Texas at Dallas, 2The NanoTech Institute, The University of Texas at Dallas, 3Department of Applied Physics, Aalto University School of Science


JoVE 52380

A method of fabricating, in ambient conditions, organic photovoltaic tandem devices in a parallel configuration is presented. These devices feature an air-processed, semi-transparent, carbon nanotube common cathode.

 JoVE Applied Physics

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

1Department of Biology, Northeastern Illinois University, 2Department of Chemistry, Northeastern Illinois University


JoVE 52164

A method for investigating the structure of a protein photoreceptor using atomic force microscopy (AFM) is described in this paper. PeakForce Quantitative Nanomechanical Property Mapping (PF-QNM) reveals intact protein dimers on a mica surface.

 JoVE Applied Physics

High-Sensitivity Nuclear Magnetic Resonance at Giga-Pascal Pressures: A New Tool for Probing Electronic and Chemical Properties of Condensed Matter under Extreme Conditions

1Faculty of Physics and Earth Sciences, University of Leipzig


JoVE 52243

Nuclear magnetic resonance is one of the most important spectroscopic tools. Here, the development of a new approach under high pressure, currently up to 10.1 GPa, is presented. This opens a new window into condensed matter physics and chemistry, where high-pressure research is of great importance.

 JoVE Applied Physics

The Preparation of Electrohydrodynamic Bridges from Polar Dielectric Liquids

1Applied Water Physics, Wetsus - Centre of Excellence for Sustainable Water Technology, 2IRCAM GmbH, 3Institute for Thermal Turbomachinery and Machine Dynamics, Graz University of Technology


JoVE 51819

Horizontal and vertical electrohydrodynamic liquid bridges are simple and powerful tools for exploring the interaction of high intensity electric fields and polar dielectric liquids. The construction of basic apparatus and operational examples, including thermographic images, for three liquids (e.g., water, DMSO, and glycerol) is presented.

 JoVE Applied Physics

Using Microwave and Macroscopic Samples of Dielectric Solids to Study the Photonic Properties of Disordered Photonic Bandgap Materials

1Department of Physics and Astronomy, San Francisco State University


JoVE 51614

Disordered structures offer new mechanisms for forming photonic bandgaps and unprecedented freedom in functional-defect designs. To circumvent the computational challenges of disordered systems, we construct modular macroscopic samples of the new class of PBG materials and use microwaves to characterize their scale-invariant photonic properties, in an easy and inexpensive manner.

 JoVE Applied Physics

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light

1ARC Centre of Excellence for Electromaterials Science (ACES), Intelligent Polymer Research Institute (IPRI), The University of Wollongong, 2School of Chemistry, The University of Sydney, 3School of Chemistry, The University of New South Wales


JoVE 52028

An integrated device, incorporating a dye-sensitized solar cell and triplet-triplet annihilation up-conversion unit was produced, affording enhanced light harvesting, from a wider section of the solar spectrum. Under modest irradiation levels a significantly enhanced response to low energy photons was demonstrated, yielding a record figure of merit for dye-sensitized solar cells.

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