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General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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JoVE Applied Physics
JoVE Applied Physics encompasses a broad range of experimental and instrumental techniques utilized in physics research. Investigations in this area strive to address and answer a broad range of scientific questions, such as device mechanisms and efficiencies, using physical tools. This approach often requires a combination of specialties, and research in this area tends to be interdisciplinary with contributions from mechanical, electrical, and chemical engineers.
 JoVE Applied Physics

The Preparation of Electrohydrodynamic Bridges from Polar Dielectric Liquids

1Applied Water Physics, Wetsus - Centre of Excellence for Sustainable Water Technology, 2IRCAM GmbH, 3Institute for Thermal Turbomachinery and Machine Dynamics, Graz University of Technology


JoVE 51819

Horizontal and vertical electrohydrodynamic liquid bridges are simple and powerful tools for exploring the interaction of high intensity electric fields and polar dielectric liquids. The construction of basic apparatus and operational examples, including thermographic images, for three liquids (e.g., water, DMSO, and glycerol) is presented.

 JoVE Applied Physics

Using Microwave and Macroscopic Samples of Dielectric Solids to Study the Photonic Properties of Disordered Photonic Bandgap Materials

1Department of Physics and Astronomy, San Francisco State University


JoVE 51614

Disordered structures offer new mechanisms for forming photonic bandgaps and unprecedented freedom in functional-defect designs. To circumvent the computational challenges of disordered systems, we construct modular macroscopic samples of the new class of PBG materials and use microwaves to characterize their scale-invariant photonic properties, in an easy and inexpensive manner.

 JoVE Applied Physics

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light

1ARC Centre of Excellence for Electromaterials Science (ACES), Intelligent Polymer Research Institute (IPRI), The University of Wollongong, 2School of Chemistry, The University of Sydney, 3School of Chemistry, The University of New South Wales


JoVE 52028

An integrated device, incorporating a dye-sensitized solar cell and triplet-triplet annihilation up-conversion unit was produced, affording enhanced light harvesting, from a wider section of the solar spectrum. Under modest irradiation levels a significantly enhanced response to low energy photons was demonstrated, yielding a record figure of merit for dye-sensitized solar cells.

 JoVE Applied Physics

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

1Institute of Applied Research, Aalen University


JoVE 51993

A module for single plane illumination microscopy (SPIM) is described which is easily adapted to an inverted wide-field microscope and optimized for 3-dimensional cell cultures. The sample is located within a rectangular capillary, and via a microfluidic system fluorescent dyes, pharmaceutical agents or drugs can be applied in small quantities.

 JoVE Applied Physics

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators

1Department of Electrical and Computer Engineering, University of California, Davis, 2Digital Light Projection (DLP) Technology Development, Texas Instruments, 3Birck Nanotechnology Center and the Department of Electrical and Computer Engineering, Purdue University


JoVE 51251

The robust device design of fringing-field electrostatic MEMS actuators results in inherently low squeeze-film damping conditions and long settling times when performing switching operations using conventional step biasing. Real-time switching time improvement with DC-dynamic waveforms reduces the settling time of fringing-field MEMS actuators when transitioning between up-to-down and down-to-up states.

 JoVE Applied Physics

Micro-masonry for 3D Additive Micromanufacturing

1Mechanical Science and Engineering, University of Illinois at Urbana-Champaign


JoVE 51974

This paper introduces a 3D additive micromanufacturing strategy (termed ‘micro-masonry’) for the flexible fabrication of microelectromechanical system (MEMS) structures and devices. This approach involves transfer printing-based assembly of micro/nanoscale materials in conjunction with rapid thermal annealing-enabled material bonding techniques.

 JoVE Applied Physics

Writing and Low-Temperature Characterization of Oxide Nanostructures

1Department of Physics, University of Pittsburgh


JoVE 51886

Oxide nanostructures provide new opportunities for science and technology. The interfacial conductivity between LaAlO3 and SrTiO3 can be controlled with near-atomic precision using a conductive atomic force microscopy technique. The protocol for creating and measuring conductive nanostructures at LaAlO3/SrTiO3 interfaces is demonstrated.

 JoVE Applied Physics

Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles

1Photonics and Optoelectronics Group, Ludwig-Maximilians-Universität


JoVE 51502

We use optical tracking of plasmonic nanoparticles to probe and characterize the frequency movements of aquatic organisms.

 JoVE Applied Physics

Coherent anti-Stokes Raman Scattering (CARS) Microscopy Visualizes Pharmaceutical Tablets During Dissolution

1Optical Sciences Group, MESA+ Institute, University of Twente, 2Institute of Pharmaceutics and Biopharmaceutics, Heinrich-Heine University, 3Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki


JoVE 51847

Coherent anti-Stokes Raman scattering (CARS) microscopy is combined with an intrinsic flow-through dissolution setup to allow in situ and real-time visualization of the surface of pharmaceutical tablets undergoing dissolution. Using this custom-built setup, it is possible to correlate CARS videos with drug dissolution profiles recorded using inline UV absorption spectroscopy.

 JoVE Applied Physics

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

1Experimental Molecular Biophysics, Freie Universität Berlin


JoVE 51622

Key steps of protein function, in particular backbone conformational changes and proton transfer reactions, often take place in the microsecond to millisecond time scale. These dynamical processes can be studied by time-resolved step-scan Fourier-transform infrared spectroscopy, in particular for proteins whose function is triggered by light.

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