Alan A. Doucette

Publications

• Maximizing Cumulative Trypsin Activity with Calcium at Elevated Temperature for Enhanced Bottom-Up Proteome Analysis
  Biology. Oct, 2022  |   Pubmed ID: 36290348
• Automated Electrokinetic Platform for High-Throughput Sodium Dodecyl Sulfate Depletion Ahead of Proteome Analysis by Mass Spectrometry
  Analytical Chemistry. 10, 2021  |   Pubmed ID: 34644045
• Recent Advances in Top-down Proteome Sample Processing Ahead of MS Analysis
  Mass Spectrometry Reviews. May, 2021  |   Pubmed ID: 34047392
• Correction To: A Robust Strategy for Proteomic Identification of Biomarkers of Invasive Phenotype Complexed with Extracellular Heat Shock Proteins
  Cell Stress & Chaperones. Mar, 2021  |   Pubmed ID: 33355877
• Mass Spectrometry Profiling of Low Molecular Weight Proteins and Peptides Isolated by Acetone Precipitation
  Analytica Chimica Acta. Nov, 2020  |   Pubmed ID: 33161983
• Rapid and Quantitative Protein Precipitation for Proteome Analysis by Mass Spectrometry
  Journal of Proteome Research. May, 2020  |   Pubmed ID: 32195589
• Developing Front-end Devices for Improved Sample Preparation in MS-based Proteome Analysis
  Journal of Mass Spectrometry : JMS. Mar, 2020  |   Pubmed ID: 31957906
• A Robust Strategy for Proteomic Identification of Biomarkers of Invasive Phenotype Complexed with Extracellular Heat Shock Proteins
  Cell Stress & Chaperones. 11, 2019  |   Pubmed ID: 31650515
• Membrane-Based SDS Depletion Ahead of Peptide and Protein Analysis by Mass Spectrometry
  Proteomics. 05, 2018  |   Pubmed ID: 29575800
• Accelerated SDS Depletion from Proteins by Transmembrane Electrophoresis: Impacts of Joule Heating
  Electrophoresis. Jun, 2018  |   Pubmed ID: 29417596
• The Benefits (and Misfortunes) of SDS in Top-down Proteomics
  Journal of Proteomics. 03, 2018  |   Pubmed ID: 28286130
• Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by Chaperone Affinity Enrichment
  Proteomes. Oct, 2017  |   Pubmed ID: 28991197
• Mass Spectrometry of Intact Proteins Reveals +98 U Chemical Artifacts Following Precipitation in Acetone
  Journal of Proteome Research. 02, 2017  |   Pubmed ID: 28088865
• Automated SDS Depletion for Mass Spectrometry of Intact Membrane Proteins Though Transmembrane Electrophoresis
  Journal of Proteome Research. 08, 2016  |   Pubmed ID: 27376408
• Preventing N- and O-formylation of Proteins when Incubated in Concentrated Formic Acid
  Proteomics. Apr, 2016  |   Pubmed ID: 26840995
• Comparison of Sodium Dodecyl Sulfate Depletion Techniques for Proteome Analysis by Mass Spectrometry
  Journal of Chromatography. A. Oct, 2015  |   Pubmed ID: 26422304
• A Two-stage Spin Cartridge for Integrated Protein Precipitation, Digestion and SDS Removal in a Comparative Bottom-up Proteomics Workflow
  Journal of Proteomics. Apr, 2015  |   Pubmed ID: 25316050
• Resolubilization of Precipitated Intact Membrane Proteins with Cold Formic Acid for Analysis by Mass Spectrometry
  Journal of Proteome Research. Dec, 2014  |   Pubmed ID: 25384094
• Reprint of "GELFrEE Fractionation Combined with Mass Spectrometry for Proteome Analysis of Secreted Toxins from Enteropathogenic Escherichia Coli (EPEC)"
  Molecular and Cellular Probes. Apr-Jun, 2014  |   Pubmed ID: 24486296
• Proteomic Analysis of Rat Proximal Tubule Cells Following Stretch-induced Apoptosis in an in Vitro Model of Kidney Obstruction
  Journal of Proteomics. Apr, 2014  |   Pubmed ID: 24316357
• Proteomic Workflows for Biomarker Identification Using Mass Spectrometry - Technical and Statistical Considerations During Initial Discovery
  Proteomes. Aug, 2013  |   Pubmed ID: 28250400
• Dual LC-MS Platform for High-throughput Proteome Analysis
  Journal of Proteome Research. Dec, 2013  |   Pubmed ID: 24090060
• Maximizing Recovery of Water-soluble Proteins Through Acetone Precipitation
  Analytica Chimica Acta. Sep, 2013  |   Pubmed ID: 24016582
• GELFrEE Fractionation Combined with Mass Spectrometry for Proteome Analysis of Secreted Toxins from Enteropathogenic Escherichia Coli (EPEC)
  Molecular and Cellular Probes. Oct-Dec, 2013  |   Pubmed ID: 23831145
• A Universal, High Recovery Assay for Protein Quantitation Through Temperature Programmed Liquid Chromatography (TPLC)
  Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Mar, 2013  |   Pubmed ID: 23435344
• Critical Assessment of the Spectroscopic Activity Assay for Monitoring Trypsin Activity in Organic-aqueous Solvent
  Analytical Biochemistry. Apr, 2013  |   Pubmed ID: 23333224
• Chromatographic Behaviour of Peptides Following Dimethylation with H2/D2-formaldehyde: Implications for Comparative Proteomics
  Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Nov, 2012  |   Pubmed ID: 23122402
• Perfluorooctanoic Acid and Ammonium Perfluorooctanoate: Volatile Surfactants for Proteome Analysis?
  Rapid Communications in Mass Spectrometry : RCM. Mar, 2012  |   Pubmed ID: 22302492
• Intact Proteome Fractionation Strategies Compatible with Mass Spectrometry
  Expert Review of Proteomics. Dec, 2011  |   Pubmed ID: 22087661
• Implications of Partial Tryptic Digestion in Organic-aqueous Solvent Systems for Bottom-up Proteome Analysis
  Analytica Chimica Acta. Oct, 2011  |   Pubmed ID: 21889634
• A Qualitative Proteome Investigation of the Sediment Portion of Human Urine: Implications in the Biomarker Discovery Process
  Proteomics. Clinical Applications. Jan, 2009  |   Pubmed ID: 21136939
• Multiplexed Size Separation of Intact Proteins in Solution Phase for Mass Spectrometry
  Analytical Chemistry. Aug, 2009  |   Pubmed ID: 19572727
• Evaluation of a Solution Isoelectric Focusing Protocol As an Alternative to Ion Exchange Chromatography for Charge-based Proteome Prefractionation
  Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Mar, 2009  |   Pubmed ID: 19246255
• Rapid and Effective Focusing in a Carrier Ampholyte Solution Isoelectric Focusing System: a Proteome Prefractionation Tool
  Journal of Proteome Research. Apr, 2008  |   Pubmed ID: 18284188
• Gel-eluted Liquid Fraction Entrapment Electrophoresis: an Electrophoretic Method for Broad Molecular Weight Range Proteome Separation
  Analytical Chemistry. Mar, 2008  |   Pubmed ID: 18229945
• Cyclic Polyamide Oligomers Extracted from Nylon 66 Membrane Filter Disks As a Source of Contamination in Liquid Chromatography/mass Spectrometry
  Journal of the American Society for Mass Spectrometry. May, 2006  |   Pubmed ID: 16517177