JoVE Immunology and Infection
Hélène Salmon1,2, Ana Rivas-Caicedo1,2, François Asperti-Boursin1,2, Camille Lebugle1,2, Pierre Bourdoncle1,2, Emmanuel Donnadieu1,2
1Institut Cochin, Université Paris Descartes, CNRS (UMR 8104), 2Inserm, U1016, Paris, France
This protocol describes a method to image fluorescent T cells introduced into lymph node slices. The technique permits real-time analyses of T cell migration with traditional widefield fluorescence or confocal microscopes.
PloS One. 2009 | Pubmed ID: 19759904
CCR7-mediated signalling is important for dendritic cell maturation and homing to the lymph nodes. We have previously demonstrated that Jak3 participates in the signalling pathway of CCR7 in T lymphocytes.