Articles by Andrew Breite in JoVE

Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease

JoVE 4137 9/07/2012

Natalie D. Stull¹, Andrew Breite², Robert McCarthy², Sarah A. Tersey¹, Raghavendra G. Mirmira^1,3,4

¹Department of Pediatrics and the Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, ²VITACYTE, LLC, ³Department of Medicine, Indiana University School of Medicine, ⁴Department of Cellular and Integrative Physiology, Indiana University School of Medicine

A detailed description of mouse islet isolation is described using the technique of in situ pancreatic ductal cannulation and perfusion of a combination of purified collagenase and neutral protease.