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In JoVE (1)
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Articles by Anna Maria Cicero in JoVE
A longo prazo de Teste de toxicidade letal com o crustáceo Artemia franciscana
Loredana Manfra1, Federica Savorelli2, Marco Pisapia1, Erika Magaletti1, Anna Maria Cicero1
1Department of Environmental Quality Monitoring, Institute for Environmental Protection and Research, 2Regional Agency for Environmental Protection in Emilia-Romagna
Este estudo diz respeito ao desenvolvimento e padronização de um protocolo metodológico valioso para determinar toxicidade a longo prazo (14 dias) letal exercida por substâncias químicas, efluentes industriais ou esgotos e líquidos amostras ambientais sobre o crustáceo de água salgada,
Other articles by Anna Maria Cicero on PubMed
Monitoring of Environmental Impact Resulting from Offshore Oil and Gas Installations in the Adriatic Sea: Preliminary Evaluations
Annali Di Chimica. Jul-Aug, 2003 | Pubmed ID: 12940603
Extracellular Carbohydrates Released by the Marine Diatoms Cylindrotheca Closterium, Thalassiosira Pseudonana and Skeletonema Costatum: Effect of P-depletion and Growth Status
The Science of the Total Environment. Dec, 2005 | Pubmed ID: 16223520
A laboratory study was performed on the extracellular production of carbohydrates by the marine diatoms Cylindrotheca closterium, Thalassiosira pseudonana and Skeletonema costatum. The investigation was aimed at elucidating the role of P-starvation and growth status on abundance and chemical characteristics of the released non-attached polysaccharides. Inorganic phosphorus depletion determined an increase of total polysaccharides in all species examined compared to nutrient-replete (complete f/2) conditions. The highest abundance of polysaccharides per unit cell was found in T. pseudonana (28.4 micromol C 10(-6) cells), followed by C. closterium (2.56 micromol C 10(-6) cells) and S. costatum (1.18 micromol C 10(-6) cells). Maximum production rates were found at the transition between exponential and stationary growth phase. Gas-chromatographic analysis of the dissolved fraction showed glucose to be the most abundant monomer in exponentially growing, P-replete cultures (81.6%, 90% and 32% as molar percentage of total aldoses in C. closterium, T. pseudonana and S. costatum, respectively). A strong reduction in glucose was found in C. closterium, but not in T. pseudonana and S. costatum, under P-depleted conditions. Species-specific variations in the amount and aldose signatures of the released polysaccharides according to nutrient status and growth conditions can provide useful insights on the production and persistence of these organic compounds in the water column.
Seasonal, Spatial and Inter-annual Variations of Trace Metals in Mussels from the Adriatic Sea: a Regional Gradient for Arsenic and Implications for Monitoring the Impact of Off-shore Activities
Chemosphere. Aug, 2008 | Pubmed ID: 18556045
Mussels are widely used as bioindicator organisms for monitoring chemical pollutants including trace metals. These elements are natural constituents in the marine environment and their basal concentrations in the organisms can be influenced by several environmental and biological factors. The aim of this work was to extend our knowledge on the natural variability of trace metals in mussels tissues, focusing on seasonal and inter-annual fluctuations in a coastal reference site of the Adriatic coast (Portonovo); a total of 39 samplings were performed during 5 years, providing an extended data-set for tissue levels of As, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, V and Zn. Concentrations of trace metals in mussels tissues revealed marked seasonal fluctuations with significant differences between various sampling years. Such fluctuations appeared mostly related to phytoplanctonic blooms and especially to reproductive cycle which exhibited a certain inter-annual shift of the gametogenesis period. Lower concentrations were measured in summer months for the majority of elements while a different seasonal cycle was observed for arsenic, not correlated with gonadic development, neither with other elements. Chemical speciation of arsenic was characterized to distinguish compounds of natural origin from those potentially reflecting an anthropogenic impact. Arsenobetaine and arsenocholine were always the predominant forms (up to 85% of total arsenic), while a significant increase of dimethylarsine and trimethylarsine oxide in spring (24% of total arsenic) might reflect the effect of phytoplanctonic bloom on both geochemistry and trophic transfer of this element. A significant inter-annual variability was observed for both the seasonal cycle and the range of values measured for all the elements, with particularly marked differences for arsenic, ranging from less than 10 to more than 40 microg/g in summer periods of different years. Data obtained on reference mussels were used to assess the impact of 41 off-shore platforms distributed along 5 nautical districts in the Northern and Central Adriatic Sea. Organisms sampled on these structures from 2001 to 2005 exhibited a certain enrichment of cadmium and zinc, probably associated to the use of anodic electrodes. Marked annual and geographical variations were measured for concentrations of arsenic in mussels of different platforms. However, the comparison with results of Portonovo, allowed to exclude the anthropogenic impact of exploitation activities and revealed a natural regional gradient of arsenic levels in mussels tissues associated to the changing influence of the Po river runoff on seawater salinity. In particular the higher concentrations measured in organisms sampled from platforms in the Central compared to Northern Adriatic confirmed a significant relationship between salinity and arsenic bioaccumulation, consistent with a role of arsenobetaine as an acquired osmolyte for mussels. The overall results confirmed the importance of natural variability when assessing the potential impact of anthropogenic activities.
Toxicity Evaluation of Diethylene Glycol and Its Combined Effects with Produced Waters of Off-shore Gas Platforms in the Adriatic Sea (Italy): Bioassays with Marine/estuarine Species
Marine Environmental Research. Dec, 2011 | Pubmed ID: 22245535
Diethylene glycol (DEG) is commonly used to dehydrate natural gas in off-shore extraction plants and to prevent formation of gas hydrates. It may be released into the sea accidentally or in discharged produced waters (PWs). PWs samples from off-shore gas platforms in the Adriatic Sea (Italy) have been used in this study. The objectives of the study were: a) to evaluate the toxicity of DEG for marine organisms; b) to evaluate if a high DEG content in PWs may alter their toxicity; c) to verify whether the DEG threshold concentration established by the Italian legislation (3.5 g/l) for PWs discharged at sea is safe for marine environment. Ten different species (Vibrio fischeri, Phaeodactylum tricornutum, Dunaliella tertiolecta, Brachionus plicatilis, Artemia franciscana, Tigropus fulvus, Mytilus galloprovincialis, Crassostrea gigas, Tapes philippinarum and Dicentrarchus labrax) have been exposed to DEG; four of these species were also exposed to PWs in combination with DEG. The results showed that: a) DEG is not toxic at levels normally detected in Adriatic PWs; b) DEG in combination with PW showed mainly additive or synergistic effects; c) short-term bioassays showed that the DEG limit of 3.5 g/l could be acceptable.