Christian Gachet Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS, F-67065 Strasbourg, France Biography Publications Institution JoVE Articles Christian Gachet has not added a biography. If you are Christian Gachet and would like to personalize this page please email our Author Liaison for assistance. Publications Platelet P2Y Receptor Deletion or Pharmacological Inhibition Does Not Protect Mice from Sepsis or Septic Shock TH Open : Companion Journal to Thrombosis and Haemostasis. Jul, 2021 | Pubmed ID: 34447900 Respective Roles of Glycoprotein VI and FcγRIIA in the Regulation of αIIbβ3-mediated Platelet Activation to Fibrinogen, Thrombus Buildup, and Stability Research and Practice in Thrombosis and Haemostasis. Jul, 2021 | Pubmed ID: 34263103 Correction: An Essential Role for α4A-tubulin in Platelet Biogenesis Life Science Alliance. Aug, 2021 | Pubmed ID: 34155037 CDX2 Regulates ACE Expression in Blood Development and Leukemia Cells Blood Advances. 04, 2021 | Pubmed ID: 33843985 Atypical Focal Segmental Glomerulosclerosis Associated with a New PODXL Nonsense Variant Molecular Genetics & Genomic Medicine. Mar, 2021 | Pubmed ID: 33780168 Human Platelets Labeled at Two Discrete Biotin Densities Are Functional in Vitro and Are Detected in Vivo in the Murine Circulation: A Promising Approach to Monitor Platelet Survival in Vivo in Clinical Research Transfusion. May, 2021 | Pubmed ID: 33580977 Removal of Citrate from PAS-III Additive Solution Improves Functional and Biochemical Characteristics of Buffy-coat Platelet Concentrates Stored for 7 days, with or Without INTERCEPT Pathogen Reduction Transfusion. Mar, 2021 | Pubmed ID: 33527430 Differential Role of Glycoprotein VI in Mouse and Human Thrombus Progression and Stability Thrombosis and Haemostasis. Apr, 2021 | Pubmed ID: 33124026 Cytoskeletal-based Mechanisms Differently Regulate and Proplatelet Formation Haematologica. May, 2021 | Pubmed ID: 32327502 Shear Rate Gradients Promote a Bi-phasic Thrombus Formation on Weak Adhesive Proteins, Such As Fibrinogen in a VWF-dependent Manner Haematologica. 10, 2020 | Pubmed ID: 33054087 Platelet δ-Storage Pool Disease: An Update Journal of Clinical Medicine. Aug, 2020 | Pubmed ID: 32759727 Pathogenic and Likely Pathogenic Variants in at Least Five Genes Account for Approximately 3% of Mild Isolated Nonsyndromic Thrombocytopenia Transfusion. 10, 2020 | Pubmed ID: 32757236 Disrupting the Platelet Internal Membrane Via PI3KC2α Inhibition Impairs Thrombosis Independently of Canonical Platelet Activation Science Translational Medicine. 07, 2020 | Pubmed ID: 32718993 Megakaryocytes Use in Vivo Podosome-like Structures Working Collectively to Penetrate the Endothelial Barrier of Bone Marrow Sinusoids Journal of Thrombosis and Haemostasis : JTH. 11, 2020 | Pubmed ID: 32702204 Pharmacological Blockade of Glycoprotein VI Promotes Thrombus Disaggregation in the Absence of Thrombin Arteriosclerosis, Thrombosis, and Vascular Biology. 09, 2020 | Pubmed ID: 32698684 Platelet Purinergic Receptors in Thrombosis and Inflammation Hamostaseologie. May, 2020 | Pubmed ID: 32464678 Update of P2Y Receptor Pharmacology: IUPHAR Review 27 British Journal of Pharmacology. 06, 2020 | Pubmed ID: 32037507 High Prevalence of the Natural Asn89Asp Mutation in the GP1BB Gene Associated with Bernard-Soulier Syndrome in French Patients from the Genetic Isolate of Reunion Island British Journal of Haematology. 05, 2020 | Pubmed ID: 31997307 Use of Electron Microscopy to Study Megakaryocytes Platelets. Jul, 2020 | Pubmed ID: 31903825 Severe Bleeding and Absent ADP-induced Platelet Aggregation Associated with Inherited Combined CalDAG-GEFI and P2Y Deficiencies Haematologica. 07, 2020 | Pubmed ID: 31649128 Platelet Preparation for Function Testing in the Laboratory and Clinic: Historical and Practical Aspects Research and Practice in Thrombosis and Haemostasis. Oct, 2019 | Pubmed ID: 31624781 Combined Deficiency of RAB32 and RAB38 in the Mouse Mimics Hermansky-Pudlak Syndrome and Critically Impairs Thrombosis Blood Advances. 08, 2019 | Pubmed ID: 31399401 Cell Surface Expression of HLA I Molecules As a Marker of Young Platelets Journal of Thrombosis and Haemostasis : JTH. 09, 2019 | Pubmed ID: 31207003 The ATP-gated P2X Ion Channel Contributes to the Severity of Antibody-mediated Transfusion-Related Acute Lung Injury in Mice Scientific Reports. 03, 2019 | Pubmed ID: 30914724 An Essential Role for α4A-tubulin in Platelet Biogenesis Life Science Alliance. 02, 2019 | Pubmed ID: 30760556 Clot Contraction Drives the Translocation of Procoagulant Platelets to Thrombus Surface Arteriosclerosis, Thrombosis, and Vascular Biology. 01, 2019 | Pubmed ID: 30580561 The PI 3-kinase PI3KC2α Regulates Mouse Platelet Membrane Structure and Function Independently of Membrane Lipid Composition FEBS Letters. 01, 2019 | Pubmed ID: 30421419 On the Way to Platelet Production Frontiers in Medicine. 2018 | Pubmed ID: 30211166 High-Resolution 3D Imaging of Megakaryocytes Using Focused Ion Beam-Scanning Electron Microscopy Methods in Molecular Biology (Clifton, N.J.). 2018 | Pubmed ID: 30171581 Three-Dimensional Culture in a Methylcellulose-Based Hydrogel to Study the Impact of Stiffness on Megakaryocyte Differentiation Methods in Molecular Biology (Clifton, N.J.). 2018 | Pubmed ID: 30171577 On the Way to in Vitro Platelet Production Transfusion Clinique Et Biologique : Journal De La Societe Francaise De Transfusion Sanguine. Sep, 2018 | Pubmed ID: 30150135 In Vitro Quality of Amotosalen-UVA Pathogen-inactivated Mini-pool Plasma Prepared from Whole Blood Stored Overnight Vox Sanguinis. Oct, 2018 | Pubmed ID: 30079963 Inherited Platelet Disorders : Management of the Bleeding Risk Transfusion Clinique Et Biologique : Journal De La Societe Francaise De Transfusion Sanguine. Sep, 2018 | Pubmed ID: 30077511 Platelet Transfusion: Current Challenges Transfusion Clinique Et Biologique : Journal De La Societe Francaise De Transfusion Sanguine. Sep, 2018 | Pubmed ID: 30037501 Platelets Expressing IgG Receptor FcγRIIA/CD32A Determine the Severity of Experimental Anaphylaxis Science Immunology. 04, 2018 | Pubmed ID: 29654057 Imaging Thrombosis with Tc-labeled RAM.1-antibody in Vivo Nuclear Medicine and Biology. 06, 2018 | Pubmed ID: 29625391 Immobilized Fibrinogen Activates Human Platelets Through Glycoprotein VI Haematologica. 05, 2018 | Pubmed ID: 29472360 The Role of Extracellular Matrix Stiffness in Megakaryocyte and Platelet Development and Function American Journal of Hematology. 03, 2018 | Pubmed ID: 29247535 A Unique Microenvironment in the Developing Liver Supports the Expansion of Megakaryocyte Progenitors Blood Advances. Sep, 2017 | Pubmed ID: 29296832 Amotosalen/UVA Pathogen Inactivation Technology Reduces Platelet Activability, Induces Apoptosis and Accelerates Clearance Haematologica. 12, 2017 | Pubmed ID: 29192131 Platelet Integrins in Tumor Metastasis: Do They Represent a Therapeutic Target? Cancers. Sep, 2017 | Pubmed ID: 28956830 Macrothrombocytopenia and Dense Granule Deficiency Associated with FLI1 Variants: Ultrastructural and Pathogenic Features Haematologica. 06, 2017 | Pubmed ID: 28255014 Dual Role of IL-21 in Megakaryopoiesis and Platelet Homeostasis Haematologica. 04, 2017 | Pubmed ID: 28057742 Platelet Integrin 61 Controls Lung Metastasis Through Direct Binding to Cancer Cell-derived ADAM9 JCI Insight. 09, 2016 | Pubmed ID: 27699237 Respective Contributions of Single and Compound Granule Fusion to Secretion by Activated Platelets Blood. 11, 2016 | Pubmed ID: 27625359 Importance of Environmental Stiffness for Megakaryocyte Differentiation and Proplatelet Formation Blood. 10, 2016 | Pubmed ID: 27503502 Inherited Dysfunctional Platelet P2Y Receptor Mutations Associated with Bleeding Disorders Hamostaseologie. Nov, 2016 | Pubmed ID: 27487748 Coagulation Factors Bound to Procoagulant Platelets Concentrate in Cap Structures to Promote Clotting Blood. 09, 2016 | Pubmed ID: 27432876 Hemostatic Properties and Protein Expression Profile of Therapeutic Apheresis Plasma Treated with Amotosalen and Ultraviolet A for Pathogen Inactivation Transfusion. 09, 2016 | Pubmed ID: 27250038 Standardization of a Well-controlled in Vivo Mouse Model of Thrombus Formation Induced by Mechanical Injury Thrombosis Research. May, 2016 | Pubmed ID: 26967532 Time-Dependent Decay of MRNA and Ribosomal RNA During Platelet Aging and Its Correlation with Translation Activity PloS One. 2016 | Pubmed ID: 26808079 [Anti-platelets Without a Bleeding Risk: Novel Targets and Strategies] Biologie Aujourd'hui. 2015 | Pubmed ID: 26820829 In Vitro Binding of [³H]PSB-0413 to P2Y₁₂ Receptors Current Protocols in Pharmacology. Dec, 2015 | Pubmed ID: 26646192 Purinergic Receptors in Thrombosis and Inflammation Arteriosclerosis, Thrombosis, and Vascular Biology. Nov, 2015 | Pubmed ID: 26359511 Platelets in Cancer. From Basic Research to Therapeutic Implications Hamostaseologie. 2015 | Pubmed ID: 26289826 Blood Cell Palmitoleate-Palmitate Ratio Is an Independent Prognostic Factor for Amyotrophic Lateral Sclerosis PloS One. 2015 | Pubmed ID: 26147510 Resolving the Multifaceted Mechanisms of the Ferric Chloride Thrombosis Model Using an Interdisciplinary Microfluidic Approach Blood. Aug, 2015 | Pubmed ID: 25931587 Dehydration of Blood Platelets by Zeodration: in Vitro Characterization and Hemostatic Properties in Vivo Transfusion. Sep, 2015 | Pubmed ID: 25856501 The Class II PI 3-kinase, PI3KC2α, Links Platelet Internal Membrane Structure to Shear-dependent Adhesive Function Nature Communications. Mar, 2015 | Pubmed ID: 25779105 NTPDase2 and the P2Y1 Receptor Are Not Required for Mammalian Eye Formation Purinergic Signalling. Mar, 2015 | Pubmed ID: 25504514 The P2X1 Receptor is Required for Neutrophil Extravasation During Lipopolysaccharide-induced Lethal Endotoxemia in Mice Journal of Immunology (Baltimore, Md. : 1950). Jan, 2015 | Pubmed ID: 25480563 Identification of a New Dysfunctional Platelet P2Y12 Receptor Variant Associated with Bleeding Diathesis Blood. Feb, 2015 | Pubmed ID: 25428217 Proteome Changes in Platelets After Pathogen Inactivation--an Interlaboratory Consensus Transfusion Medicine Reviews. Apr, 2014 | Pubmed ID: 24685438 FLow-induced PRotrusions (FLIPRs): a Platelet-derived Platform for the Retrieval of Microparticles by Monocytes and Neutrophils Circulation Research. Feb, 2014 | Pubmed ID: 24406984 Contractile Forces Sustain and Polarize Hematopoiesis from Stem and Progenitor Cells Cell Stem Cell. Jan, 2014 | Pubmed ID: 24268694 Biogenesis of the Demarcation Membrane System (DMS) in Megakaryocytes Blood. Feb, 2014 | Pubmed ID: 24152908 Involvement of Glial P2Y₁ Receptors in Cognitive Deficit After Focal Cerebral Stroke in a Rodent Model Journal of Neuroinflammation. Jul, 2013 | Pubmed ID: 23890321 Integrin α6β1 is the Main Receptor for Vascular Laminins and Plays a Role in Platelet Adhesion, Activation, and Arterial Thrombosis Circulation. Jul, 2013 | Pubmed ID: 23797810 Astrocytes Protect Neurons Against Methylmercury Via ATP/P2Y(1) Receptor-mediated Pathways in Astrocytes PloS One. 2013 | Pubmed ID: 23469098 Preserved Functional and Biochemical Characteristics of Platelet Components Prepared with Amotosalen and Ultraviolet A for Pathogen Inactivation Transfusion. Jun, 2013 | Pubmed ID: 23067365 Purinergic Signaling Promotes Proliferation of Adult Mouse Subventricular Zone Cells The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jul, 2012 | Pubmed ID: 22764232 The Extent of P2Y12 Inhibition by Clopidogrel in Diabetes Mellitus Patients with Acute Coronary Syndrome is Not Related to Glycaemic Control: Roles of White Blood Cell Count and Body Weight Thrombosis and Haemostasis. Aug, 2012 | Pubmed ID: 22740070 P2Y(12) Receptors in Platelets and Other Hematopoietic and Non-hematopoietic Cells Purinergic Signalling. Sep, 2012 | Pubmed ID: 22528678 Association of Estimated GFR with Platelet Inhibition in Patients Treated with Clopidogrel American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation. Jun, 2012 | Pubmed ID: 22425260 Romiplostim Administration Shows Reduced Megakaryocyte Response-capacity and Increased Myelofibrosis in a Mouse Model of MYH9-RD Blood. Apr, 2012 | Pubmed ID: 22234693 P2 Receptors and Platelet Function Purinergic Signalling. Sep, 2011 | Pubmed ID: 21792575 Major Contribution of the P2Y₁receptor in Purinergic Regulation of TNFα-induced Vascular Inflammation Circulation. May, 2011 | Pubmed ID: 21576651 The Antithrombotic Activity of EP224283, a Neutralizable Dual Factor Xa Inhibitor/glycoprotein IIbIIIa Antagonist, Exceeds That of the Coadministered Parent Compounds The Journal of Pharmacology and Experimental Therapeutics. Aug, 2011 | Pubmed ID: 21527535 Comparison of Two Murine Models of Thrombosis Induced by Atherosclerotic Plaque Injury Thrombosis and Haemostasis. May, 2011 | Pubmed ID: 21479341 Cardiovascular Mortality in Chronic Kidney Disease Patients Undergoing Percutaneous Coronary Intervention is Mainly Related to Impaired P2Y12 Inhibition by Clopidogrel Journal of the American College of Cardiology. Jan, 2011 | Pubmed ID: 21251579 Impact of P2Y12 Inhibition by Clopidogrel on Cardiovascular Mortality in Unselected Patients Treated by Percutaneous Coronary Angioplasty: a Prospective Registry JACC. Cardiovascular Interventions. Jun, 2010 | Pubmed ID: 20630458 A Central Role of GPIb-IX in the Procoagulant Function of Platelets That is Independent of the 45-kDa GPIbalpha N-terminal Extracellular Domain Blood. Aug, 2010 | Pubmed ID: 20457869 Intrinsic Impaired Proplatelet Formation and Microtubule Coil Assembly of Megakaryocytes in a Mouse Model of Bernard-Soulier Syndrome Haematologica. Jun, 2009 | Pubmed ID: 19377075 In Vitro and In Vivo Methods to Explore Megakaryopoiesis François Lanza1, Christian Gachet1, Anita Eckly1 1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS, F-67065 Strasbourg, France JoVE 63286 Editorial In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy Cyril Scandola1, François Lanza1, Christian Gachet1, Anita Eckly1 1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS JoVE 62494 Biology Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement Julie Boscher1, Christian Gachet1, François Lanza1, Catherine Léon1 1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS JoVE 62511 Biology In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow Alicia Bornert1, Fabien Pertuy1, François Lanza1, Christian Gachet1, Catherine Léon1 1INSERM, EFS Grand Est, Université de Strasbourg JoVE 62515 Biology Proplatelet Formation Dynamics of Mouse Fresh Bone Marrow Explants Inès Guinard1, François Lanza1, Christian Gachet1, Catherine Léon1, Anita Eckly1 1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS JoVE 62501 Biology Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation Anaïs Pongerard1, Lea Mallo1, Christian Gachet1, Henri de La Salle1, François Lanza1, Catherine Strassel1 1BPPS UMR-S 1255, FMTS, Université de Strasbourg, INSERM, EFS Grand Est JoVE 62499 Biology Isolation of Mouse Megakaryocyte Progenitors Quentin Kimmerlin1, Manuela Tavian2, Christian Gachet1, François Lanza1, Nathalie Brouard1 1Université de Strasbourg, INSERM UMR S1255, EFS Grand-EST, 2UMR-S1113 –IRFAC, Université de Strasbourg JoVE 62498 Biology
In Vitro and In Vivo Methods to Explore Megakaryopoiesis François Lanza1, Christian Gachet1, Anita Eckly1 1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS, F-67065 Strasbourg, France JoVE 63286 Editorial
In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy Cyril Scandola1, François Lanza1, Christian Gachet1, Anita Eckly1 1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS JoVE 62494 Biology
Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement Julie Boscher1, Christian Gachet1, François Lanza1, Catherine Léon1 1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS JoVE 62511 Biology
In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow Alicia Bornert1, Fabien Pertuy1, François Lanza1, Christian Gachet1, Catherine Léon1 1INSERM, EFS Grand Est, Université de Strasbourg JoVE 62515 Biology
Proplatelet Formation Dynamics of Mouse Fresh Bone Marrow Explants Inès Guinard1, François Lanza1, Christian Gachet1, Catherine Léon1, Anita Eckly1 1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS JoVE 62501 Biology
Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation Anaïs Pongerard1, Lea Mallo1, Christian Gachet1, Henri de La Salle1, François Lanza1, Catherine Strassel1 1BPPS UMR-S 1255, FMTS, Université de Strasbourg, INSERM, EFS Grand Est JoVE 62499 Biology
Isolation of Mouse Megakaryocyte Progenitors Quentin Kimmerlin1, Manuela Tavian2, Christian Gachet1, François Lanza1, Nathalie Brouard1 1Université de Strasbourg, INSERM UMR S1255, EFS Grand-EST, 2UMR-S1113 –IRFAC, Université de Strasbourg JoVE 62498 Biology
