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JoVE Immunology and Infection Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry JoVE 2050 7/08/2010 Christina Farr1, Stuart Berger1,2 1Immunology, University of Toronto, 2Arthritis and Immune Disorder Research Centre, University Health Network (UHN) This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.