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- Met behulp van de GELFREE 8100 Fractionering System for Molecular Weight-Based Fractionering met Liquid Phase Recovery
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Articles by Chuck Witkowski in JoVE
Met behulp van de GELFREE 8100 Fractionering System for Molecular Weight-Based Fractionering met Liquid Phase Recovery
Chuck Witkowski, Jay Harkins
Research and Development, Protein Discovery, Inc.
De bijhorende video beschrijft het gebruik van de GELFREE 8100 Fractionation System, welke partities complex eiwit monsters op basis van moleculair gewicht en herstelt de fracties in de vloeibare fase. De video beschrijft hoe de technologie werkt, hoe het wordt gebruikt, en biedt resulterende data, met polyacrylamidegelelektroforese analyse van gefractioneerde runderen leverhomogenaat.
Other articles by Chuck Witkowski on PubMed
Direct Sampling and Analysis from Solid-phase Extraction Cards Using an Automated Liquid Extraction Surface Analysis Nanoelectrospray Mass Spectrometry System
Rapid Communications in Mass Spectrometry : RCM. Sep, 2011 | Pubmed ID: 21793068
Direct liquid extraction based surface sampling, a technique previously demonstrated with continuous flow and autonomous pipette liquid microjunction surface sampling probes, has recently been implemented as a liquid extraction surface analysis (LESA) mode on a commercially available chip-based infusion nanoelectrospray ionization (nanoESI) system. In the present paper, the LESA mode was applied to the analysis of 96-well format custom-made solid-phase extraction (SPE) cards, with each well consisting of either a 1 or a 2 mm diameter monolithic hydrophobic stationary phase. These substrate wells were conditioned, loaded with either single or multi-component aqueous mixtures, and read out using the commercial nanoESI system coupled to a hybrid triple quadrupole/linear ion trap mass spectrometer or a linear ion trap mass spectrometer. The extraction conditions, including extraction/nanoESI solvent composition, volume, and dwell times, were optimized in the analysis of targeted compounds. Limit of detection and quantitation as well as analysis reproducibility figures of merit were measured. Calibration data was obtained for propranolol using a deuterated internal standard which demonstrated linearity and reproducibility. A 10× increase in signal and cleanup of micromolar angiotensin II from a concentrated salt solution was demonstrated. In addition, a multicomponent herbicide mixture at ppb concentration levels was analyzed using MS(3) spectra for compound identification in the presence of isobaric interferences.