Craig R. Roy Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine Yale University School of Medicine Biography Publications Institution JoVE Articles Craig R. Roy has not added a biography. If you are Craig R. Roy and would like to personalize this page please email our Author Liaison for assistance. Publications Analysis of Dot/Icm Type IVB Secretion System Subassemblies by Cryoelectron Tomography Reveals Conformational Changes Induced by DotB Binding MBio. Feb, 2020 | Pubmed ID: 32071271 Biogenesis of the Spacious -Containing Vacuole Depends on Host Transcription Factors TFEB and TFE3 Infection and Immunity. Feb, 2020 | Pubmed ID: 31818957 Screening Effectors for Antiviral Effects Reveals Rab1 GTPase As a Proviral Factor Coopted for Tombusvirus Replication Proceedings of the National Academy of Sciences of the United States of America. Oct, 2019 | Pubmed ID: 31591191 Host Cell Depletion of Tryptophan by IFNγ-induced Indoleamine 2,3-dioxygenase 1 (IDO1) Inhibits Lysosomal Replication of Coxiella Burnetii PLoS Pathogens. 08, 2019 | Pubmed ID: 31461509 Remodels the Plasma Membrane-derived Vacuole by Utilizing Exocyst Components As Tethers The Journal of Cell Biology. 11, 2018 | Pubmed ID: 30275106 A Unique Cytoplasmic ATPase Complex Defines the Legionella Pneumophila Type IV Secretion Channel Nature Microbiology. 06, 2018 | Pubmed ID: 29784975 Dot/Icm-Translocated Proteins Important for Biogenesis of the Coxiella Burnetii-Containing Vacuole Identified by Screening of an Effector Mutant Sublibrary Infection and Immunity. 04, 2018 | Pubmed ID: 29339460 Subversion of Host Membrane Dynamics by the Legionella Dot/Icm Type IV Secretion System Current Topics in Microbiology and Immunology. 2017 | Pubmed ID: 29536361 Multiple Effector Virulence Phenotypes Revealed Through High-throughput Analysis of Targeted Mutant Libraries Proceedings of the National Academy of Sciences of the United States of America. 11, 2017 | Pubmed ID: 29133401 DNA Delivery and Genomic Integration into Mammalian Target Cells Through Type IV A and B Secretion Systems of Human Pathogens Frontiers in Microbiology. 2017 | Pubmed ID: 28878740 A Farnesylated Coxiella Burnetii Effector Forms a Multimeric Complex at the Mitochondrial Outer Membrane During Infection Infection and Immunity. 05, 2017 | Pubmed ID: 28242621 Autophagic Targeting and Avoidance in Intracellular Bacterial Infections Current Opinion in Microbiology. 02, 2017 | Pubmed ID: 27984783 MTOR-Driven Metabolic Reprogramming Regulates Legionella Pneumophila Intracellular Niche Homeostasis PLoS Pathogens. Dec, 2016 | Pubmed ID: 27942021 Autophagy Evasion and Endoplasmic Reticulum Subversion: The Yin and Yang of Legionella Intracellular Infection Annual Review of Microbiology. 09, 2016 | Pubmed ID: 27607556 Erratum for Kohler Et Al., Effector Protein Cig2 Decreases Host Tolerance of Infection by Directing Constitutive Fusion of Autophagosomes with the Coxiella-Containing Vacuole MBio. 09, 2016 | Pubmed ID: 27601576 Legionella Pneumophila Type IV Effectors YlfA and YlfB Are SNARE-Like Proteins That Form Homo- and Heteromeric Complexes and Enhance the Efficiency of Vacuole Remodeling PloS One. 2016 | Pubmed ID: 27459495 Effector Protein Cig2 Decreases Host Tolerance of Infection by Directing Constitutive Fusion of Autophagosomes with the Coxiella-Containing Vacuole MBio. 07, 2016 | Pubmed ID: 27435465 Chitinase 3-Like 1 (Chil1) Regulates Survival and Macrophage-Mediated Interleukin-1β and Tumor Necrosis Factor Alpha During Pseudomonas Aeruginosa Pneumonia Infection and Immunity. 07, 2016 | Pubmed ID: 27141083 Primary Role for Toll-Like Receptor-Driven Tumor Necrosis Factor Rather Than Cytosolic Immune Detection in Restricting Coxiella Burnetii Phase II Replication Within Mouse Macrophages Infection and Immunity. Apr, 2016 | Pubmed ID: 26787725 Inhibition of Inflammasome Activation by Coxiella Burnetii Type IV Secretion System Effector IcaA Nature Communications. 2015 | Pubmed ID: 26687278 The Legionella Anti-autophagy Effector RavZ Targets the Autophagosome Via PI3P- and Curvature-Sensing Motifs Developmental Cell. Sep, 2015 | Pubmed ID: 26343456 Biogenesis of the Lysosome-derived Vacuole Containing Coxiella Burnetii Microbes and Infection. Nov-Dec, 2015 | Pubmed ID: 26327296 Structure and Function of Fic Proteins Nature Reviews. Microbiology. Oct, 2015 | Pubmed ID: 26299785 Toxicity and SidJ-Mediated Suppression of Toxicity Require Distinct Regions in the SidE Family of Legionella Pneumophila Effectors Infection and Immunity. Sep, 2015 | Pubmed ID: 26099583 On the Use of Legionella/Rickettsia Chimeras to Investigate the Structure and Regulation of Rickettsia Effector RalF Journal of Structural Biology. Feb, 2015 | Pubmed ID: 25498244 Alveolar Macrophages and Neutrophils Are the Primary Reservoirs for Legionella Pneumophila and Mediate Cytosolic Surveillance of Type IV Secretion Infection and Immunity. Oct, 2014 | Pubmed ID: 25092908 A Screen of Coxiella Burnetii Mutants Reveals Important Roles for Dot/Icm Effectors and Host Autophagy in Vacuole Biogenesis PLoS Pathogens. Jul, 2014 | Pubmed ID: 25080348 The Machinery at Endoplasmic Reticulum-plasma Membrane Contact Sites Contributes to Spatial Regulation of Multiple Legionella Effector Proteins PLoS Pathogens. Jul, 2014 | Pubmed ID: 24992562 Structural Basis for PI(4)P-specific Membrane Recruitment of the Legionella Pneumophila Effector DrrA/SidM Structure (London, England : 1993). Mar, 2014 | Pubmed ID: 24530282 AMPylation is Critical for Rab1 Localization to Vacuoles Containing Legionella Pneumophila MBio. Feb, 2014 | Pubmed ID: 24520063 Legionella Pneumophila Subversion of Host Vesicular Transport by SidC Effector Proteins Traffic (Copenhagen, Denmark). May, 2014 | Pubmed ID: 24483784 A Novel Membrane Sensor Controls the Localization and ArfGEF Activity of Bacterial RalF PLoS Pathogens. 2013 | Pubmed ID: 24244168 Pathogen Signatures Activate a Ubiquitination Pathway That Modulates the Function of the Metabolic Checkpoint Kinase MTOR Nature Immunology. Dec, 2013 | Pubmed ID: 24121838 A Rab-centric Perspective of Bacterial Pathogen-occupied Vacuoles Cell Host & Microbe. Sep, 2013 | Pubmed ID: 24034612 Autophagy and Bacterial Infection: an Evolving Arms Race Trends in Microbiology. Sep, 2013 | Pubmed ID: 23880062 Identification and Functional Characterization of K(+) Transporters Encoded by Legionella Pneumophila Kup Genes Cellular Microbiology. Dec, 2013 | Pubmed ID: 23848378 The Legionella Pneumophila GTPase Activating Protein LepB Accelerates Rab1 Deactivation by a Non-canonical Hydrolytic Mechanism The Journal of Biological Chemistry. Aug, 2013 | Pubmed ID: 23821544 Structure of the Legionella Effector AnkX Reveals the Mechanism of Phosphocholine Transfer by the FIC Domain The EMBO Journal. May, 2013 | Pubmed ID: 23572077 Host Pathways Important for Coxiella Burnetii Infection Revealed by Genome-wide RNA Interference Screening MBio. 2013 | Pubmed ID: 23362322 Effector Protein Translocation by the Coxiella Burnetii Dot/Icm Type IV Secretion System Requires Endocytic Maturation of the Pathogen-occupied Vacuole PloS One. 2013 | Pubmed ID: 23349930 Caspase-11 Stimulates Rapid Flagellin-independent Pyroptosis in Response to Legionella Pneumophila Proceedings of the National Academy of Sciences of the United States of America. Jan, 2013 | Pubmed ID: 23307811 Analyzing Caspase-1 Activation During Legionella Pneumophila Infection in Macrophages Methods in Molecular Biology (Clifton, N.J.). 2013 | Pubmed ID: 23150415 The Role of Rab GTPases in the Transport of Vacuoles Containing Legionella Pneumophila and Coxiella Burnetii Biochemical Society Transactions. Dec, 2012 | Pubmed ID: 23176480 The Capping Domain in RalF Regulates Effector Functions PLoS Pathogens. 2012 | Pubmed ID: 23166491 The Legionella Effector RavZ Inhibits Host Autophagy Through Irreversible Atg8 Deconjugation Science (New York, N.Y.). Nov, 2012 | Pubmed ID: 23112293 Coxiella Burnetii Secretion Systems Advances in Experimental Medicine and Biology. 2012 | Pubmed ID: 22711632 Vacuolar Pathogens Value Membrane Integrity Proceedings of the National Academy of Sciences of the United States of America. Feb, 2012 | Pubmed ID: 22343287 The Legionella Pneumophila Effector DrrA is Sufficient to Stimulate SNARE-dependent Membrane Fusion Cell Host & Microbe. Jan, 2012 | Pubmed ID: 22264512 Subversion of Membrane Transport Pathways by Vacuolar Pathogens The Journal of Cell Biology. Dec, 2011 | Pubmed ID: 22123831 The Coxiella Burnetii Dot/Icm System Creates a Comfortable Home Through Lysosomal Renovation MBio. 2011 | Pubmed ID: 22010216 Modulation of Rab GTPase Function by a Protein Phosphocholine Transferase Nature. Aug, 2011 | Pubmed ID: 21822290 Dissection of a Type I Interferon Pathway in Controlling Bacterial Intracellular Infection in Mice Cellular Microbiology. Nov, 2011 | Pubmed ID: 21790939 Asc Modulates the Function of NLRC4 in Response to Infection of Macrophages by Legionella Pneumophila MBio. 2011 | Pubmed ID: 21771913 The Coxiella Burnetii Dot/Icm System Delivers a Unique Repertoire of Type IV Effectors into Host Cells and is Required for Intracellular Replication PLoS Pathogens. May, 2011 | Pubmed ID: 21637816 Inhibition of Pathogen-induced Apoptosis by a Coxiella Burnetii Type IV Effector Protein Proceedings of the National Academy of Sciences of the United States of America. Nov, 2010 | Pubmed ID: 20944063 Lipidation by the Host Prenyltransferase Machinery Facilitates Membrane Localization of Legionella Pneumophila Effector Proteins The Journal of Biological Chemistry. Nov, 2010 | Pubmed ID: 20813839 Manipulation of Host Membrane Machinery by Bacterial Pathogens Current Opinion in Cell Biology. Aug, 2010 | Pubmed ID: 20542678 Cooperation Between Multiple Microbial Pattern Recognition Systems is Important for Host Protection Against the Intracellular Pathogen Legionella Pneumophila Infection and Immunity. Jun, 2010 | Pubmed ID: 20351139 The Anaplasma Phagocytophilum-occupied Vacuole Selectively Recruits Rab-GTPases That Are Predominantly Associated with Recycling Endosomes Cellular Microbiology. Sep, 2010 | Pubmed ID: 20345488 Legionella Pneumophila Promotes Functional Interactions Between Plasma Membrane Syntaxins and Sec22b Traffic (Copenhagen, Denmark). May, 2010 | Pubmed ID: 20163564 Rapid Pathogen-induced Apoptosis: a Mechanism Used by Dendritic Cells to Limit Intracellular Replication of Legionella Pneumophila PLoS Pathogens. Jun, 2009 | Pubmed ID: 19521510 Structure and Function of Interacting IcmR-IcmQ Domains from a Type IVb Secretion System in Legionella Pneumophila Structure (London, England : 1993). Apr, 2009 | Pubmed ID: 19368892 Bacterial FIC Proteins AMP Up Infection Science Signaling. Mar, 2009 | Pubmed ID: 19293428 Asc and Ipaf Inflammasomes Direct Distinct Pathways for Caspase-1 Activation in Response to Legionella Pneumophila Infection and Immunity. May, 2009 | Pubmed ID: 19237518 A Legionella Pneumophila Effector Protein Encoded in a Region of Genomic Plasticity Binds to Dot/Icm-modified Vacuoles PLoS Pathogens. Jan, 2009 | Pubmed ID: 19165328 Modulation of Ubiquitin Dynamics and Suppression of DALIS Formation by the Legionella Pneumophila Dot/Icm System Cellular Microbiology. Feb, 2009 | Pubmed ID: 19016782 Multiple MyD88-dependent Responses Contribute to Pulmonary Clearance of Legionella Pneumophila Cellular Microbiology. Jan, 2009 | Pubmed ID: 18782351 Type IV Secretion-dependent Activation of Host MAP Kinases Induces an Increased Proinflammatory Cytokine Response to Legionella Pneumophila PLoS Pathogens. Nov, 2008 | Pubmed ID: 19043549 Ankyrin Repeat Proteins Comprise a Diverse Family of Bacterial Type IV Effectors Science (New York, N.Y.). Jun, 2008 | Pubmed ID: 18566289 Analyzing Association of the Endoplasmic Reticulum with the Legionella Pneumophila-containing Vacuoles by Fluorescence Microscopy Methods in Molecular Biology (Clifton, N.J.). 2008 | Pubmed ID: 18425463 Host Cell Processes That Influence the Intracellular Survival of Legionella Pneumophila Cellular Microbiology. Jun, 2008 | Pubmed ID: 18363881 The Legionella Pneumophila IcmSW Complex Interacts with Multiple Dot/Icm Effectors to Facilitate Type IV Translocation PLoS Pathogens. Dec, 2007 | Pubmed ID: 18069892 Legionella Pneumophila Proteins That Regulate Rab1 Membrane Cycling Nature. Nov, 2007 | Pubmed ID: 17952054 Pathogen Subversion of Cell-intrinsic Innate Immunity Nature Immunology. Nov, 2007 | Pubmed ID: 17952043 Coxiella Burnetii Inhibits Activation of Host Cell Apoptosis Through a Mechanism That Involves Preventing Cytochrome C Release from Mitochondria Infection and Immunity. Nov, 2007 | Pubmed ID: 17709406 Effector Proteins Translocated by Legionella Pneumophila: Strength in Numbers Trends in Microbiology. Aug, 2007 | Pubmed ID: 17632005 NALP3: a Key Player in Caspase-1 Activation Journal of Endotoxin Research. 2006 | Pubmed ID: 16953978 The Legionella Pneumophila Effector Protein DrrA is a Rab1 Guanine Nucleotide-exchange Factor Nature Cell Biology. Sep, 2006 | Pubmed ID: 16906144 Recognition and Delivery of Effector Proteins into Eukaryotic Cells by Bacterial Secretion Systems Traffic (Copenhagen, Denmark). Aug, 2006 | Pubmed ID: 16734660 MyD88-dependent Responses Involving Toll-like Receptor 2 Are Important for Protection and Clearance of Legionella Pneumophila in a Mouse Model of Legionnaires' Disease Infection and Immunity. Jun, 2006 | Pubmed ID: 16714560 Attachment and Fusion of Endoplasmic Reticulum with Vacuoles Containing Legionella Pneumophila Cellular Microbiology. May, 2006 | Pubmed ID: 16611228 Flagellin-deficient Legionella Mutants Evade Caspase-1- and Naip5-mediated Macrophage Immunity PLoS Pathogens. Mar, 2006 | Pubmed ID: 16552444 Pathogen-endoplasmic-reticulum Interactions: in Through the out Door Nature Reviews. Immunology. Feb, 2006 | Pubmed ID: 16491138 The Birc1e Cytosolic Pattern-recognition Receptor Contributes to the Detection and Control of Legionella Pneumophila Infection Nature Immunology. Mar, 2006 | Pubmed ID: 16444259 Activated Macrophages Infected with Legionella Inhibit T Cells by Means of MyD88-dependent Production of Prostaglandins Journal of Immunology (Baltimore, Md. : 1950). Dec, 2005 | Pubmed ID: 16339557 Effect of Vitrector Cutting Rate on Vitreous Biopsy Yield Retina (Philadelphia, Pa.). Sep, 2005 | Pubmed ID: 16141874 A Yeast Genetic System for the Identification and Characterization of Substrate Proteins Transferred into Host Cells by the Legionella Pneumophila Dot/Icm System Molecular Microbiology. May, 2005 | Pubmed ID: 15853880 Processing and Major Histocompatibility Complex Class II Presentation of Legionella Pneumophila Antigens by Infected Macrophages Infection and Immunity. Apr, 2005 | Pubmed ID: 15784579 Bacterial Subversion of the Host Secretory Pathway Proceedings of the National Academy of Sciences of the United States of America. Feb, 2005 | Pubmed ID: 15677328 The Legionella IcmS-IcmW Protein Complex is Important for Dot/Icm-mediated Protein Translocation Molecular Microbiology. Feb, 2005 | Pubmed ID: 15661013 A C-terminal Translocation Signal Required for Dot/Icm-dependent Delivery of the Legionella RalF Protein to Host Cells Proceedings of the National Academy of Sciences of the United States of America. Jan, 2005 | Pubmed ID: 15613486 Stimulation of Toll-like Receptor 2 by Coxiella Burnetii is Required for Macrophage Production of Pro-inflammatory Cytokines and Resistance to Infection The Journal of Biological Chemistry. Dec, 2004 | Pubmed ID: 15485838 Immunity to Vacuolar Pathogens: What Can We Learn from Legionella? Cellular Microbiology. Nov, 2004 | Pubmed ID: 15469430 Legionella Subvert the Functions of Rab1 and Sec22b to Create a Replicative Organelle The Journal of Experimental Medicine. May, 2004 | Pubmed ID: 15117975 Legionella Reveal Dendritic Cell Functions That Facilitate Selection of Antigens for MHC Class II Presentation Immunity. Jun, 2003 | Pubmed ID: 12818162 Show Me the Substrates: Modulation of Host Cell Function by Type IV Secretion Systems Cellular Microbiology. Jun, 2003 | Pubmed ID: 12780775 Eukaryotic Cells and Microbial Pathogens: a Familiar Couple Take Centre Stage Nature Cell Biology. Jan, 2003 | Pubmed ID: 12511887 Legionella Phagosomes Intercept Vesicular Traffic from Endoplasmic Reticulum Exit Sites Nature Cell Biology. Dec, 2002 | Pubmed ID: 12447391 Exploitation of the Endoplasmic Reticulum by Bacterial Pathogens Trends in Microbiology. Sep, 2002 | Pubmed ID: 12217507 The Road Less Traveled: Transport of Legionella to the Endoplasmic Reticulum The Journal of Cell Biology. Aug, 2002 | Pubmed ID: 12147677 The Dot/lcm Transporter of Legionella Pneumophila: a Bacterial Conductor of Vesicle Trafficking That Orchestrates the Establishment of a Replicative Organelle in Eukaryotic Hosts International Journal of Medical Microbiology : IJMM. Feb, 2002 | Pubmed ID: 11890545 A Bacterial Guanine Nucleotide Exchange Factor Activates ARF on Legionella Phagosomes Science (New York, N.Y.). Jan, 2002 | Pubmed ID: 11809974 The DotA Protein from Legionella Pneumophila is Secreted by a Novel Process That Requires the Dot/Icm Transporter The EMBO Journal. Nov, 2001 | Pubmed ID: 11689436 Identification and Subcellular Localization of the Legionella Pneumophila IcmX Protein: a Factor Essential for Establishment of a Replicative Organelle in Eukaryotic Host Cells Infection and Immunity. Jul, 2000 | Pubmed ID: 10858211 Anwendung von Live Cell Imaging und Cryo-Electron Tomography zur Auflösung der raumzeitlichen Merkmale des Legionella pneumophila Dot/Icm Sekretionssystems David Chetrit1, Donghyun Park1,2, Bo Hu3, Jun Liu1,2, Craig R. Roy1 1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, 2Microbial Sciences Institute, Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston JoVE 60693 Immunology and Infection
Anwendung von Live Cell Imaging und Cryo-Electron Tomography zur Auflösung der raumzeitlichen Merkmale des Legionella pneumophila Dot/Icm Sekretionssystems David Chetrit1, Donghyun Park1,2, Bo Hu3, Jun Liu1,2, Craig R. Roy1 1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, 2Microbial Sciences Institute, Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston JoVE 60693 Immunology and Infection
