Articles by Dan O. Wang in JoVE

Preparation of Aplysia Sensory-motor Neuronal Cell Cultures

JoVE 1355 6/08/2009

Yali Zhao¹, Dan O. Wang¹, Kelsey C. Martin^1,2,3

¹Dept. of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles, ²Dept. of Biological Chemistry, University of California, Los Angeles, ³Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles

Primary cultures of Aplysia sensory-motor neurons provide a model preparation for studying synapse formation and synaptic plasticity in vitro. This video demonstrates the identification and microdissection of sensory and motor neurons from Aplysia ganglia as well as the methods for establishing and maintaining sensory-motor neurons in culture.