Articles by Daniel S. Dohle in JoVE

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

JoVE 1620 11/30/2009

Daniel S. Dohle¹, Susanne D. Pasa¹, Sebastian Gustmann², Markus Laub³, Josef H. Wissler⁴, Herbert P. Jennissen¹, Nicole Dünker²

¹Institute for Physiological Chemistry, Department of Biochemical Endocrinology, University of Duisburg-Essen, ²Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen, ³Morphoplant GmbH, ⁴ARCONS Institute for Applied Research and Didactics

The chick chorioallantoic membrane (CAM) is a unique, naturally immunodeficient supportive culture environment to study angiogenesis and tumorigenesis. This video article demonstrates the different steps in chick ex ovo culture, application of potentially angiogenic substances and successful inoculation of tumor cells and tissues on the surface of the CAM.