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In JoVE (1)
Other Publications (1)
Articles by Danilo Maddalo in JoVE
JoVE General
Intracellular Refolding Assay
Institute of Toxicology and Genetics, Karlsruhe Institute of Technology
In this protocol a method to measure intracellular protein refolding after heat shock is described. This method can be used to study foldases like molecular chaperones and their co-factors or compounds able to influence their activity. Firefly luciferase activity is used as reporter to measure chaperone refolding activity.
Other articles by Danilo Maddalo on PubMed
The Anterior Gradient 2 (AGR2) Gene is Overexpressed in Prostate Cancer and May Be Useful As a Urine Sediment Marker for Prostate Cancer Detection
AGR2 is a member of the endoplasmatic reticulum protein disulphide isomerase gene family implicated in tumor metastasis. Its expression pattern, function, and utility as a marker remains to be further investigated.
