Translate this page to:
In JoVE (1)
Other Publications (1)
Articles by Danilo Maddalo in JoVE
Intracellular Refolding Assay
Tamara Vanessa Walther, Danilo Maddalo
Institute of Toxicology and Genetics, Karlsruhe Institute of Technology
In this protocol a method to measure intracellular protein refolding after heat shock is described. This method can be used to study foldases like molecular chaperones and their co-factors or compounds able to influence their activity. Firefly luciferase activity is used as reporter to measure chaperone refolding activity.
Other articles by Danilo Maddalo on PubMed
The Anterior Gradient 2 (AGR2) Gene is Overexpressed in Prostate Cancer and May Be Useful As a Urine Sediment Marker for Prostate Cancer Detection
The Prostate. May, 2011 | Pubmed ID: 20945500
AGR2 is a member of the endoplasmatic reticulum protein disulphide isomerase gene family implicated in tumor metastasis. Its expression pattern, function, and utility as a marker remains to be further investigated.