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In JoVE (1)
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Articles by Danilo Maddalo in JoVE
JoVE General
Intracellulaire hervouwing Assay
Institute of Toxicology and Genetics, Karlsruhe Institute of Technology
In dit protocol een methode om intracellulair eiwit hervouwing te meten na de heat shock wordt beschreven. Deze methode kan gebruikt worden om foldases zoals moleculaire chaperonnes en hun co-factoren of verbindingen in staat om hun activiteiten van invloed te bestuderen. Vuurvlieg luciferase activiteit wordt gebruikt als reporter voor chaperonne hervouwing activiteit te meten.
Other articles by Danilo Maddalo on PubMed
The Anterior Gradient 2 (AGR2) Gene is Overexpressed in Prostate Cancer and May Be Useful As a Urine Sediment Marker for Prostate Cancer Detection
AGR2 is a member of the endoplasmatic reticulum protein disulphide isomerase gene family implicated in tumor metastasis. Its expression pattern, function, and utility as a marker remains to be further investigated.
