Articles by Deborah A. Blake in JoVE

FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability

JoVE 3289 8/05/2011

Deborah A. Blake¹, Nicolai V. Bovin², Dan Bess¹, Stephen M. Henry¹

¹Biotechnology Research Institute, AUT University and KODE Biotech Ltd, ²Shemyakin Institute of Bioorganic Chemistry RAS, Moscow, Russia

Function-Spacer-Lipid (FSL) constructs allow the surface characteristics of living cells and virions to be modified without loss of vitality. The method requires only simple contact of an FSL construct solution with a cell/virion and spontaneous and stable surface incorporation occurs.