Articles by Duke Geem in JoVE

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

JoVE 4040 5/21/2012

Duke Geem¹, Oscar Medina-Contreras¹, Wooki Kim², Clifton S. Huang¹, Timothy L. Denning^1,2

¹Department of Pediatrics, Emory University, ²Department of Pathology & Laboratory Medicine, Emory University

Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.