Articles by E. Scott Colvin in JoVE

Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets

JoVE 4080 6/25/2012

Patrick T. Fueger^1,2, Angelina M. Hernandez², Yi-Chun Chen², E. Scott Colvin^1,2

¹Department of Pediatrics, Indiana University School of Medicine, ²Department of Cellular & Integrative Physiology, Indiana University School of Medicine

This protocol allows one to identify factors that modulate functional beta cell mass to find potential therapeutic targets for the treatment of diabetes. The protocol consists of a streamlined method to assess islet replication and beta cell function in isolated rat islets following manipulation of gene expression with adenoviruses.