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In JoVE (2)
- Black Widow Spider İpek üreten Bezler, Mikrodisseksiyon
- Bir Laboratuvar Ölçekli Sentetik Örümcek İpek Üretimi
Other Publications (1)
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Articles by Felicia Jeffery in JoVE
Black Widow Spider İpek üreten Bezler, Mikrodisseksiyon
Felicia Jeffery*, Coby La Mattina*, Tiffany Tuton-Blasingame*, Yang Hsia, Eric Gnesa, Liang Zhao, Andreas Franz, Craig Vierra
Department of Biological Sciences, University of the Pacific
Burada kadın kara dul örümcekleri karın ipek üreten bezlerin kaldırmak için etkili bir strateji açıklanmaktadır. Bu prosedür, yedi ayrı ipek üreten, son derece saf bir moda, örümcek ipeği üretimi ve lif montaj inceleyen araştırmacılar için önemli bir süreci bezlerinin hızlı bir izolasyon sağlar.
Bir Laboratuvar Ölçekli Sentetik Örümcek İpek Üretimi
Yang Hsia, Eric Gnesa, Ryan Pacheco, Kristin Kohler, Felicia Jeffery, Craig Vierra
Department of Biological Sciences, University of the Pacific
Örümcek ipek ve üstün mekanik özellikleri ve biyokimyasal rağmen, bu malzeme geleneksel yollarla, büyük miktarlarda hasat edilemez. Burada nesil Biyomalzeme olarak örümcek ipeği üretimi ve kullanımı eğitimi araştırmacılar için önemli bir süreç olan yapay örümcek ipeği lifleri, spin etkili bir strateji tanımlamak.
Other articles by Felicia Jeffery on PubMed
Synthetic Spider Silk Fibers Spun from Pyriform Spidroin 2, a Glue Silk Protein Discovered in Orb-weaving Spider Attachment Discs
Biomacromolecules. Dec, 2010 | Pubmed ID: 21053953
Spider attachment disc silk fibers are spun into a viscous liquid that rapidly solidifies, gluing dragline silk fibers to substrates for locomotion or web construction. Here we report the identification and artificial spinning of a novel attachment disc glue silk fibroin, Pyriform Spidroin 2 (PySp2), from the golden orb weaver Nephila clavipes . MS studies support PySp2 is a constituent of the pyriform gland that is spun into attachment discs. Analysis of the PySp2 protein architecture reveals sequence divergence relative to the other silk family members, including the cob weaver glue silk fibroin PySp1. PySp2 contains internal block repeats that consist of two subrepeat units: one dominated by Ser, Gln, and Ala and the other Pro-rich. Artificial spinning of recombinant PySp2 truncations shows that the Ser-Gln-Ala-rich subrepeat is sufficient for the assembly of polymeric subunits and subsequent fiber formation. These studies support that both orb- and cob-weaving spiders have evolved highly polar block-repeat sequences with the ability to self-assemble into fibers, suggesting a strategy to allow fiber fabrication in the liquid environment of the attachment discs.
