Articles by Frederic Leblond in JoVE

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

JoVE 4050 7/17/2012

Kenneth M. Tichauer¹, Robert W. Holt², Kimberley S. Samkoe³, Fadi El-Ghussein¹, Jason R. Gunn¹, Michael Jermyn¹, Hamid Dehghani⁴, Frederic Leblond¹, Brian W. Pogue^1,2

¹Thayer School of Engineering, Dartmouth College, ²Department of Physics and Astronomy, Dartmouth College, ³Darmouth Medical School, Dartmouth College, ⁴School of Computer Science, University of Birmingham

Diffuse fluorescence tomography offers a relatively low-cost and potentially high-throughout approach to preclinical in vivo tumor imaging. The methodology of optical data collection, calibration, and image reconstruction is presented for a computed tomography-guided non-contact time-domain system using fluorescent targeting of the tumor biomarker epidermal growth factor receptor in a mouse glioma model.