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In JoVE (1)
Other Publications (6)
Articles by Giusi M. Bellistri in JoVE
JoVE Clinical and Translational Medicine
Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients
Our experiment will show how to perform a sequencing analysis of bacterial species translocating in peripheral blood of HIV positive patients.
Other articles by Giusi M. Bellistri on PubMed
Microbial Translocation is Associated with Sustained Failure in CD4+ T-cell Reconstitution in HIV-infected Patients on Long-term Highly Active Antiretroviral Therapy
Patients with inefficient CD4+ T-cell recovery on virogically suppressive highly active antiretroviral therapy constitute a major clinical hurdle given the threat of HIV/AIDS disease progression. We show heightened circulating lipopolysaccharide associated with plasma enterobacterial DNA and highly activated Ki67+CD4+CD8+ in 24 immunologic-nonresponders (CD4+ T-cell < or = 200; HIV-RNA < or = 50) compared with 11 full responders (CD4+ T-cell > or= 400; HIV-RNA < or = 50). These data provide novel insight into INRs pathogenesis, since they correlate augmented systemic translocation of microbial bioproducts with T-cell hyperactivation.
Abacavir and Cardiovascular Risk in HIV-infected Patients: Does T Lymphocyte Hyperactivation Exert a Pathogenic Role?
Sudden Cardiac Death in a Young HIV-positive Man on Effective Antiretroviral Therapy
We describe the case of a young HIV-positive man on effective HAART with excellent viro-immunological control who presented a massive cardiac infarction. Despite the presence of clinical risk factors for cardiovascular disease, the patient had normal arterial carotid IMT values, known to be strong predictors of atherosclerosis and stroke. Interestingly, parameters of T-cell activation (CD8+CD38+) were shown to increase just before the onset of myocardial infarction. As T-cell activation is known to mediate atherosclerosis, the authors suggest that surrogate immunologic markers should be identified to better assess cardiovascular risk in the setting of HIV infection.
CD8+ Hyperactivation and Senescence Correlate with Early Carotid Intima-media Thickness in HIV+ Patients with No Cardiovascular Disease
Reduced CD127 Expression on Peripheral CD4+ T Cells Impairs Immunological Recovery in Course of Suppressive Highly Active Antiretroviral Therapy
Inefficient immune recovery under highly active antiretroviral therapy (HAART) represents a clinical issue. Twenty-seven of 121 HIV+ naïve patients became immunological nonresponders (INRs) and 55 introduced therapy late [very late treated (VLT)]. INR displayed older age, lower CD4(+) cell counts, down-regulation of CD127(+)CD4(+) and higher apoptotic CD95(+)CD8(+). VLT also showed higher activated CD38(+)CD8(+)%. The only factor associated with INR status was CD127(+)CD4(+)%. INR showed lower baseline interleukin (IL)-7 levels and a reduced expression of IL-7R (CD127(+)) on naïve and memory T-cells, reaching significance in memory CD127(+)CD45(+)R0(+)CD4(+). These results suggest a possible role for the IL-7/IL-7R system in the pathogenesis of poor immunological recovery during HAART.
Microbial Translocation Predicts Disease Progression of HIV-infected Antiretroviral-naive Patients with High CD4+ Cell Count
We investigated the significance of microbial translocation measured on average 3 years after HIV seroconversion in driving disease progression in HIV untreated patients with high CD4(+) cell count.
