Articles by Guangchao Sui in JoVE

DNA Vector-based RNA Interference to Study Gene Function in Cancer

JoVE 4129 6/04/2012

Daniel B. Stovall¹, Meimei Wan¹, Qiang Zhang¹, Purnima Dubey², Guangchao Sui¹

¹Department of Cancer Biology and Comprehensive Cancer Center, Wake Forest University School of Medicine, ²Department of Pathology and Comprehensive Cancer Center, Wake Forest University School of Medicine

RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in vivo.