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In JoVE (1)
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Articles by Hannah A. Arnson in JoVE
JoVE Neuroscience
Многоэлектродной массива Записи вомероназального Эпителий
Anatomy and Neurobiology, Washington University School of Medicine
Многоэлектродной массива (МЭС) записи обеспечивают метод для изучения электрической активности больших популяций нейронов. Здесь мы представляем подробности подготовки МЭС для записи с мышью вомероназального эпителия одновременно стимулирует ткани.
Other articles by Hannah A. Arnson on PubMed
Representation and Transformation of Sensory Information in the Mouse Accessory Olfactory System
In mice, nonvolatile social cues are detected and analyzed by the accessory olfactory system (AOS). Here we provide a first view of information processing in the AOS with respect to individual chemical cues. 12 sulfated steroids, recently discovered mouse AOS ligands, caused widespread activity among vomeronasal sensory neurons (VSNs), yet VSN responses clustered into a small number of repeated functional patterns or processing streams. Downstream neurons in the accessory olfactory bulb (AOB) responded to these ligands with enhanced signal/noise compared to VSNs. Although the dendritic connectivity of AOB mitral cells suggests the capacity for broad integration, most sulfated steroid responses were well-modeled by linear excitatory drive from just one VSN processing stream. However, a substantial minority demonstrated multi-stream integration. Most VSN excitation patterns were also observed in the AOB, but excitation by estradiol sulfate processing streams was rare, suggesting AOB circuit organization is specific to the biological relevance of sensed cues.
Chemosensory Burst Coding by Mouse Vomeronasal Sensory Neurons
The capabilities of any sensory system are ultimately constrained by the properties of the sensory neurons: the ability to detect and represent stimuli is limited by noise due to spontaneous activity, and optimal decoding in downstream circuitry must be matched to the nature of the encoding performed at the input. Here, we investigated the firing properties of sensory neurons in the accessory olfactory system, a distinct sensory system specialized for detection of socially relevant odors. Using multielectrode array recording, we observed that sensory neurons are spontaneously active and highly variable across time and trials and that this spontaneous activity limits the ability to distinguish sensory responses from noise. Sensory neuron activity tended to consist of bursts that maintained remarkably consistent statistics during both spontaneous activity and in response to stimulation with sulfated steroids. This, combined with pharmacological and genetic intervention in the signal transduction cascade, indicates that sensory transduction plays a role in shaping overall spontaneous activity. These findings indicate that as-yet unexplored characteristics of the sensory transduction cascade significantly constrain the representation of sensory information by vomeronasal neurons.
