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In JoVE (1)
Other Publications (1)
Articles by Heather L. Machado in JoVE
Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
Kevin J. Zwezdaryk*1, Jessica A. Warner*1,2, Heather L. Machado3, Cindy A. Morris1, Kerstin Höner zu Bentrup1
1Department of Microbiology and Immunology, Tulane University Medical School, 2Physician/Scientist Program, Tulane University Medical School, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
Traditional, two dimensional cell culture techniques often result in altered characteristics with respect to differentiation markers, cytokines and growth factors. Three-dimensional cell culture in the rotating cell culture system (RCCS) reestablishes expression of many of these factors as shown here with an extravillous trophoblast cell line.
Other articles by Heather L. Machado on PubMed
PloS One. 2011 | Pubmed ID: 22140470
Stem cell antigen-1 (Sca-1 or Ly6A) is a glycosyl phostidylinositol (GPI)-anchored cell surface protein associated with both stem and progenitor activity, as well as tumor initiating-potential. However, at present the functional role for Sca-1 is poorly defined.