Translate this page to:
Swedish
In JoVE (1)
Other Publications (2)
Automatic Translation
This translation into Swedish was automatically generated.
English Version | Other Languages
Articles by Jason Saunders in JoVE
JoVE Clinical and Translational Medicine
En hög kapacitet automatiserad plattform för utveckling av tillverkning cellinjer för proteinläkemedel
Merck Research Laboratory, Merck & Co., Inc
En hög genomströmning, automatiserad plattform för tillverkning cellinje utveckling för produktion av proteinläkemedel beskrivs. Genomförande av BD FACS Aria Cell Sorter har CloneSelect Imager och TECAN Freedom EVO vätskehantering systemet visade signifikant ökad förädling kapaciteten i cellinje utveckling med ökad cellinje kvalitet och hög reproducerbarhet.
Other articles by Jason Saunders on PubMed
The Combination of Electrically Stimulated Gracilis Neoanal Sphincter and Continent Colonic Conduit: a Step Forward for Total Anorectal Reconstruction?
Patients undergoing total anorectal reconstruction for anorectal atresia or following abdominoperineal resection of the rectum do not fare as well after an electrically stimulated gracilis neoanal sphincter as patients with incontinence alone. This retrospective study reports the outcome for the combination of a continent colonic conduit or antegrade continence enema procedure with an electrically stimulated gracilis neoanal sphincter in patients with atresia or following an abdominoperineal resection of the rectum as part of total anorectal reconstruction to overcome combined incontinence and evacuatory dysfunction.
Repression of Cardiac Phospholamban Gene Expression is Mediated by Thyroid Hormone Receptor-{alpha}1 and Involves Targeted Covalent Histone Modifications
Phospholamban (PLB) is a critical regulator of Ca(2+) cycling in heart muscle cells, and its gene expression is markedly down-regulated by T(3). Nonetheless, little is known about the molecular mechanisms of T(3)-dependent gene silencing in cardiac muscle, and it remains unclear whether thyroid hormone receptors (TRs) directly bind at the PLB gene in vivo and facilitate transcriptional repression. To investigate the regulatory role of TRs in PLB transcription, we used a physiological murine heart muscle cell line (HL-1) that retains cardiac electrophysiological properties, expresses both TRalpha1 and TRbeta1 subtypes, and exhibits T(3)-dependent silencing of PLB expression. By performing RNA interference assays with HL-1 cells, we found that TRalpha1, but not TRbeta1, is essential for T(3)-dependent PLB gene repression. Interestingly, a PLB reporter gene containing only the core promoter sequences -156 to +64 displayed robust T(3)-dependent silencing in HL-1 cells, thus suggesting that transcriptional repression is facilitated by TRalpha1 via the PLB core promoter, a regulatory region highly conserved in mammals. Consistent with this notion, chromatin immunoprecipitation and in vitro binding assays show that TRalpha1 directly binds at the PLB core promoter region. Furthermore, addition of T(3) triggered alterations in covalent histone modifications at the PLB promoter that are associated with gene silencing, namely a pronounced decrease in both histone H3 acetylation and histone H3 lysine 4 methylation. Taken together, our data reveal that T(3)-dependent repression of PLB in cardiac myocytes is directly facilitated by TRalpha1 and involves the hormone-dependent recruitment of histone-modifying enzymes associated with transcriptional silencing.
