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In JoVE (4)
- تشريح الإنسان عناصر الجسم الزجاجي لتحليل البروتين
- نزع الأحشاء من الشبكية والسائل الزجاجي ماوس للتحليلات البروتين
- سمل العيون الماوس لPhenotyping عالي الإنتاجية عن بعد
- تحت الشبكية حقن المتجهات العلاج الجيني والخلايا الجذعية في العين ماوس
Other Publications (9)
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Articles by Jessica M. Skeie in JoVE
JoVE Clinical and Translational Medicine
تشريح الإنسان عناصر الجسم الزجاجي لتحليل البروتين
Department of Ophthalmology and Visual Sciences, Omics Laboratory, University of Iowa
هذا الفيديو يظهر تقنية فعالة للتمييز وتشريح مختلف هياكل شبه شفافة من الجسم الزجاجي في العين البشرية بعد الوفاة.
JoVE General
نزع الأحشاء من الشبكية والسائل الزجاجي ماوس للتحليلات البروتين
1Omics Laboratory, University of Iowa, 2Ophthalmology and Visual Sciences, University of Iowa, 3Harkness Eye Institute, Columbia University College of Physicians and Surgeons
تقنية يوضح تشريح استئصاله من الجسم الزجاجي والشبكية ، وعدسة العين من الماوس ، والفصل عن طريق الطرد المركزي ، وتوصيف المقايسات مع البروتين.
JoVE Clinical and Translational Medicine
سمل العيون الماوس لPhenotyping عالي الإنتاجية عن بعد
1Department of Ophthalmology and Visual Sciences, University of Iowa, 2Omics Laboratory, University of Iowa, 3School of Dentistry, UCLA, 4Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, College of Physicians and Surgeons, Columbia University
تقنية تشريح يوضح قلع العين الماوس لتثبيت الأنسجة لأداء phenotyping في شاشات عالية الإنتاجية.
JoVE Clinical and Translational Medicine
تحت الشبكية حقن المتجهات العلاج الجيني والخلايا الجذعية في العين ماوس
1Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, Columbia University, 2Institute of Human Nutrition, College of Physicians & Surgeons, Columbia University, 3Omics Laboratory, University of Iowa, 4Department of Ophthalmology and Visual Sciences, University of Iowa
هذه التقنية الجراحية يوضح حقن ناقلات العلاج الجيني والخلايا الجذعية في الفضاء تحت الشبكية للعين الماوس.
Other articles by Jessica M. Skeie on PubMed
Glycoconjugates of Choroidal Neovascular Membranes in Age-related Macular Degeneration
Choroidal neovascularization (CNV) is a complication of multiple eye diseases, including age-related macular degeneration, that usually results in irreversible vision loss. It is characterized by proliferation and growth of choroidal blood vessels through Bruch's membrane into the subpigment epithelial and/or subretinal space. The purpose of this study was to characterize the carbohydrate groups associated with CNV by lectin histochemistry.
Macular and Peripheral Distribution of ICAM-1 in the Human Choriocapillaris and Retina
In order to understand the extent of choriocapillary endothelial cell activation in different topographic regions of the eye, we sought to compare the localization of intercellular adhesion molecule-1 (ICAM-1) in macular and peripheral regions of human eyes.
Comparison of the Femtosecond Laser (IntraLase) Versus Manual Microkeratome (Moria ALTK) in Dissection of the Donor in Endothelial Keratoplasty: Initial Study in Eye Bank Eyes
To determine the safety and efficacy of a femtosecond laser (IntraLase) and manual microkeratome (Moria ALTK) in creating precut endothelial keratoplasty donor tissue.
Elastin-mediated Choroidal Endothelial Cell Migration: Possible Role in Age-related Macular Degeneration
Endothelial cell (EC) migration is a key event in angiogenesis, and is likely to play an important role in choroidal neovascularization in age-related macular degeneration (AMD). Altered elastin metabolism has been described in AMD, and the present study sought to determine the effects of elastin-derived peptides (EDPs) on choroidal EC migration and proliferation.
Complement Component C5a Activates ICAM-1 Expression on Human Choroidal Endothelial Cells
The complement system plays a crucial role in the progression of age-related macular degeneration (AMD). In this study, the authors sought to evaluate the pathophysiologic roles of complement components C3a and C5a in the human choroid in AMD.
Angiogenin in Age-related Macular Degeneration
Age-related macular degeneration (AMD) is a common blinding disease in the elderly population. AMD is frequently complicated by choroidal neovascularization, causing irreversible losses in visual acuity. Proteins that induce pathologic angiogenesis in other systems include angiogenin, a small protein involved in angiogenesis in tumor metastases. Our goal was to determine if angiogenin participates in angiogenesis during choroidal neovascular membrane formation in AMD.
Choriocapillaris Vascular Dropout Related to Density of Drusen in Human Eyes with Early Age-related Macular Degeneration
Age-related macular degeneration (AMD) is a common, potentially blinding disease characterized by the presence of extracellular deposits beneath the retinal pigment epithelium (RPE). Choroidal vascular changes have also been noted in AMD. This study examined the relationship between the choroidal vasculature and extent of drusen and other sub-RPE deposits, the key pathologic landmarks of AMD.
Evaluation of Variants in the Selectin Genes in Age-related Macular Degeneration
Age-related macular degeneration (AMD) is a common disease of the elderly that leads to loss of the central visual field due to atrophic or neovascular events. Evidence from human eyes and animal models suggests an important role for macrophages and endothelial cell activation in the pathogenesis of AMD. We sought to determine whether common ancestral variants in genes encoding the selectin family of proteins are associated with AMD.
Molecular Responses of Choroidal Endothelial Cells to Elastin Derived Peptides Through the Elastin-binding Protein (GLB1)
PURPOSE: Neovascular AMD involves the activation of choroidal endothelial cells to increase their inflammatory and angiogenic behaviors. Elastin derived peptides (EDPs) can elicit some of these phenotypic changes in endothelial cells. This investigation was performed to follow up on those findings by determining a receptor for these peptides in the human eye as well as evaluating the effects of elevated EDPs on choroidal cells in vitro and in vivo. METHODS: The expression of elastin receptor genes including GLB1 was analyzed using reverse transcription PCR. Migration of choroidal endothelial cells was quantified in the presence of inhibitors to different EDP binding proteins. C57BL6 mice were injected with EDPs and studied by electroretinography, transmission electron microscopy, and microarray analysis. RESULTS: An alternatively spliced form of beta-galactosidase (GLB1) is present on human choroidal endothelial cells and acts as a receptor for EDPs. Elevated levels of circulating EDPs do not affect retinal function in the mouse, but do increase the expression and deposition of collagen IV in the RPE/choroid complex. CONCLUSIONS: EDPs may play a role in neovascular AMD by binding to and inducing neovascular phenotypes in choroidal endothelial cells through their receptor, GLB1. These peptides also cause an increased mRNA expression and deposition of collagen IV in the RPE/choroid, which may alter diffusion properties between the retina and choriocapillaris.
