Kapil Bharti Ocular and Stem Cell Translational Research Section, National Eye Institute National Institutes of Health (NIH) Biography Publications Institution JoVE Articles Kapil Bharti has not added a biography. If you are Kapil Bharti and would like to personalize this page please email our Author Liaison for assistance. Publications Considerations for Developing an Autologous Induced Pluripotent Stem Cell (iPSC)-Derived Retinal Pigment Epithelium (RPE) Replacement Therapy Cold Spring Harbor Perspectives in Medicine. Jul, 2023 | Pubmed ID: 37487631 Retina and RPE Lipid Profile Changes Linked with ABCA4 Associated Stargardt's Maculopathy Pharmacology & Therapeutics. Jun, 2023 | Pubmed ID: 37385300 Protocol to Generate Endothelial Cells, Pericytes, and Fibroblasts in One Differentiation Round from Human-induced Pluripotent Stem Cells STAR Protocols. May, 2023 | Pubmed ID: 37149860 Stem Cell Sources and Characterization in the Development of Cell-based Products for Treating Retinal Disease: An NEI Town Hall Report Stem Cell Research & Therapy. Mar, 2023 | Pubmed ID: 36978104 Bioprinted 3D Outer Retina Barrier Uncovers RPE-dependent Choroidal Phenotype in Advanced Macular Degeneration Nature Methods. Jan, 2023 | Pubmed ID: 36550275 Cell-autonomous Lipid-handling Defects in Stargardt IPSC-derived Retinal Pigment Epithelium Cells Stem Cell Reports. Nov, 2022 | Pubmed ID: 36306781 Triphasic Developmentally Guided Protocol to Generate Retinal Pigment Epithelium from Induced Pluripotent Stem Cells STAR Protocols. Sep, 2022 | Pubmed ID: 35880133 Cell Culture Models to Study Retinal Pigment Epithelium-related Pathogenesis in Age-related Macular Degeneration Experimental Eye Research. Sep, 2022 | Pubmed ID: 35835183 In vitro Disease Modeling of Oculocutaneous Albinism Type 1 and 2 Using Human Induced Pluripotent Stem Cell-derived Retinal Pigment Epithelium Stem Cell Reports. Jan, 2022 | Pubmed ID: 35021041 Epithelial Phenotype Restoring Drugs Suppress Macular Degeneration Phenotypes in an IPSC Model Nature Communications. Dec, 2021 | Pubmed ID: 34911940 AMPK Modulation Ameliorates Dominant Disease Phenotypes of CTRP5 Variant in Retinal Degeneration Communications Biology. Dec, 2021 | Pubmed ID: 34887495 CSF1R Blockade Induces Macrophage Ablation and Results in Mouse Choroidal Vascular Atrophy and RPE Disorganization ELife. Apr, 2020 | Pubmed ID: 32234210 Retinal Pigment Epithelium Replacement Therapy for Age-Related Macular Degeneration: Are We There Yet? Annual Review of Pharmacology and Toxicology. 01, 2020 | Pubmed ID: 31914900 Retinal Stem Cell Transplantation: Balancing Safety and Potential Progress in Retinal and Eye Research. 03, 2020 | Pubmed ID: 31494256 High-yield, Automated Intracellular Electrophysiology in Retinal Pigment Epithelia Journal of Neuroscience Methods. 12, 2019 | Pubmed ID: 31562888 Partial Rescue of Ocular Pigment Cells and Structure by Inducible Ectopic Expression of Mitf-M in MITF-Deficient Mice Investigative Ophthalmology & Visual Science. 12, 2018 | Pubmed ID: 30590377 Identifying Core Biological Processes Distinguishing Human Eye Tissues with Precise Systems-level Gene Expression Analyses and Weighted Correlation Networks Human Molecular Genetics. 10, 2018 | Pubmed ID: 30239781 Report of the International Conference on Manufacturing and Testing of Pluripotent Stem Cells Biologicals : Journal of the International Association of Biological Standardization. Nov, 2018 | Pubmed ID: 30150108 Allogeneic IPSC-Derived RPE Cell Graft Failure Following Transplantation Into the Subretinal Space in Nonhuman Primates Investigative Ophthalmology & Visual Science. 03, 2018 | Pubmed ID: 29625461 Primary Cilium-Mediated Retinal Pigment Epithelium Maturation Is Disrupted in Ciliopathy Patient Cells Cell Reports. 01, 2018 | Pubmed ID: 29298421 Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma Membranes Methods in Molecular Biology (Clifton, N.J.). 2018 | Pubmed ID: 29264809 MITF - A Controls Branching Morphogenesis and Nephron Endowment PLoS Genetics. 12, 2017 | Pubmed ID: 29240767 A Switchable Positive and Negative Air Pressure Device for Efficient and Gentle Handling of Nanofiber Scaffolds The Review of Scientific Instruments. Oct, 2017 | Pubmed ID: 29092483 A Basis for Comparison: Sensitive Authentication of Stem Cell Derived RPE Using Physiological Responses of Intact RPE Monolayers Stem Cell and Translational Investigation. 2017 | Pubmed ID: 28286868 Training to Improve Precision and Accuracy in the Measurement of Fiber Morphology PloS One. 2016 | Pubmed ID: 27907145 In Pursuit of Authenticity: Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium for Clinical Applications Stem Cells Translational Medicine. Jul, 2016 | Pubmed ID: 27400791 Nanofiber Scaffold-Based Tissue-Engineered Retinal Pigment Epithelium to Treat Degenerative Eye Diseases Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics. Jun, 2016 | Pubmed ID: 27110730 Human Adult Retinal Pigment Epithelial Stem Cell-Derived RPE Monolayers Exhibit Key Physiological Characteristics of Native Tissue Investigative Ophthalmology & Visual Science. Nov, 2015 | Pubmed ID: 26540654 Dataset for the Validation and Use of DiameterJ an Open Source Nanofiber Diameter Measurement Tool Data in Brief. Dec, 2015 | Pubmed ID: 26380840 Small-molecule-directed, Efficient Generation of Retinal Pigment Epithelium from Human Pluripotent Stem Cells Proceedings of the National Academy of Sciences of the United States of America. Sep, 2015 | Pubmed ID: 26269569 DiameterJ: A Validated Open Source Nanofiber Diameter Measurement Tool Biomaterials. Aug, 2015 | Pubmed ID: 26043061 PAX6 Regulates Melanogenesis in the Retinal Pigmented Epithelium Through Feed-forward Regulatory Interactions with MITF PLoS Genetics. 2014 | Pubmed ID: 24875170 Developing Cellular Therapies for Retinal Degenerative Diseases Investigative Ophthalmology & Visual Science. Feb, 2014 | Pubmed ID: 24573369 The Indirect Role of Fibroblast Growth Factor-8 in Defining Neurogenic Niches of the Olfactory/GnRH Systems The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Dec, 2013 | Pubmed ID: 24336726 Efficient and Rapid Derivation of Primitive Neural Stem Cells and Generation of Brain Subtype Neurons from Human Pluripotent Stem Cells Stem Cells Translational Medicine. Nov, 2013 | Pubmed ID: 24113065 Vax1/2 Genes Counteract Mitf-induced Respecification of the Retinal Pigment Epithelium PloS One. 2013 | Pubmed ID: 23555005 Conditional Knockdown of DNA Methyltransferase 1 Reveals a Key Role of Retinal Pigment Epithelium Integrity in Photoreceptor Outer Segment Morphogenesis Development (Cambridge, England). Mar, 2013 | Pubmed ID: 23406904 A Regulatory Loop Involving PAX6, MITF, and WNT Signaling Controls Retinal Pigment Epithelium Development PLoS Genetics. Jul, 2012 | Pubmed ID: 22792072 Lack of the Ventral Anterior Homeodomain Transcription Factor VAX1 Leads to Induction of a Second Pituitary Development (Cambridge, England). Mar, 2011 | Pubmed ID: 21247964 The New Paradigm: Retinal Pigment Epithelium Cells Generated from Embryonic or Induced Pluripotent Stem Cells Pigment Cell & Melanoma Research. Feb, 2011 | Pubmed ID: 20846177 The Basic-helix-loop-helix-leucine Zipper Gene Mitf: Analysis of Alternative Promoter Choice and Splicing Methods in Molecular Biology (Clifton, N.J.). 2010 | Pubmed ID: 20694671 Lack of the Mesodermal Homeodomain Protein MEOX1 Disrupts Sclerotome Polarity and Leads to a Remodeling of the Cranio-cervical Joints of the Axial Skeleton Developmental Biology. Aug, 2009 | Pubmed ID: 19520072 Expression Profiling During Ocular Development Identifies 2 Nlz Genes with a Critical Role in Optic Fissure Closure Proceedings of the National Academy of Sciences of the United States of America. Feb, 2009 | Pubmed ID: 19171890 Alternative Promoter Use in Eye Development: the Complex Role and Regulation of the Transcription Factor MITF Development (Cambridge, England). Mar, 2008 | Pubmed ID: 18272592 Src-dependent Phosphorylation of ASAP1 Regulates Podosomes Molecular and Cellular Biology. Dec, 2007 | Pubmed ID: 17893324 The Other Pigment Cell: Specification and Development of the Pigmented Epithelium of the Vertebrate Eye Pigment Cell Research. Oct, 2006 | Pubmed ID: 16965267 When Pigment Cells Turn into Neurons The Journal of Investigative Dermatology. Oct, 2005 | Pubmed ID: 16185257 Unraveling the Pathogenesis of Age-Related Macular Degeneration and Discovering Potential Therapies (Video) | JoVE Zander Esh*1, Sharanya Suresh*1, Davide Ortolan1, Mitra Farnoodian1, Devika Bose1, Jiwon Ryu1, Andrei Volkov1, Kapil Bharti1, Ruchi Sharma1 1Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health (NIH) JoVE 65503 Biology Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis Davide Ortolan1, Andrei Volkov1, Arvydas Maminishkis1, Ruchi Sharma1, Kapil Bharti1 1Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health (NIH) JoVE 64761 Biology Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium Kiyoharu J. Miyagishima1, Congxiao Zhang*2, Volha V. Malechka*3, Kapil Bharti2, Wei Li1 1Retinal Neurophysiology Section, National Eye Institute, National Institutes of Health, 2Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health, 3Human Visual Function Core, National Eye Institute, National Institutes of Health JoVE 61491 Chemistry
Unraveling the Pathogenesis of Age-Related Macular Degeneration and Discovering Potential Therapies (Video) | JoVE Zander Esh*1, Sharanya Suresh*1, Davide Ortolan1, Mitra Farnoodian1, Devika Bose1, Jiwon Ryu1, Andrei Volkov1, Kapil Bharti1, Ruchi Sharma1 1Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health (NIH) JoVE 65503 Biology
Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis Davide Ortolan1, Andrei Volkov1, Arvydas Maminishkis1, Ruchi Sharma1, Kapil Bharti1 1Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health (NIH) JoVE 64761 Biology
Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium Kiyoharu J. Miyagishima1, Congxiao Zhang*2, Volha V. Malechka*3, Kapil Bharti2, Wei Li1 1Retinal Neurophysiology Section, National Eye Institute, National Institutes of Health, 2Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health, 3Human Visual Function Core, National Eye Institute, National Institutes of Health JoVE 61491 Chemistry
