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In JoVE (1)
Other Publications (3)
Articles by Katherine E. Pelch in JoVE
JoVE Clinical and Translational Medicine
Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue
1Obstetrics, Gynecology and Women’s Health and Division of Biological Sciences, University of Missouri, 2Obstetrics, Gynecology and Women’s Health and Animal Sciences, University of Missouri
A description of the surgical induction of endometriosis in mice and rats by auto-transplantation of uterine tissue to the arterial cascade of the intestinal mesentery.
Other articles by Katherine E. Pelch on PubMed
Estrogen Modulates Expression of Putative Housekeeping Genes in the Mouse Uterus
Estrogens regulate gene expression and cell proliferation in target tissues. In studies of estrogen-regulated gene expression, identification of appropriate housekeeping genes (HKGs), reference genes whose expression is not altered by treatment, is difficult. The goal of this study was to define HKGs unaltered by estrogen in the mouse uterus. Ovariectomized C57BL6 mice were dosed with 20 micrograms/kg ethinylestradiol and the uterus was collected at 6, 24, and 72 h later to bracket the biphasic time course of estrogen action in the rodent uterus. RNA was isolated, cDNA synthesized and equal amounts of cDNA were added to real-time PCR reactions. The expression of seven out of nine putative HKGs was altered by estrogen in the mouse uterus. Estrogen induced four gene expression profiles, expression of: (1) Actb and Hsp90ab1 were up-regulated early, (2) B2m and Gusb were up-regulated late, (3) Gapdh, Hprt1, and Ppia were up regulated at all time points, and (4) Rpl13a and 18srRNA were unaltered. This highlights the need to empirically determine the appropriate HKG for each experimental condition. Based on these results, we suggest using Rpl13a or 18srRNA as HKGs for xenoestrogen studies in the mouse uterus and as good candidates to test under different experimental conditions.
Aberrant Gene Expression Profile in a Mouse Model of Endometriosis Mirrors That Observed in Women
To define the altered gene expression profile of endometriotic lesions in a mouse model of surgically induced endometriosis.
Developmental Exposure to Low Dose Xenoestrogens Alters Femur Length and Tensile Strength in Adult Mice
Developmental exposure to high doses of the synthetic xenoestrogen diethylstilbestrol (DES) has been reported to alter femur length and strength in adult mice. However, it is not known if developmental exposure to low, environmentally relevant doses of xenoestrogens alter adult bone geometry and strength. In this study we investigated the effects of developmental exposure to low doses of DES, bisphenol A (BPA), or ethinyl estradiol (EE(2)) on bone geometry and torsional strength. C57BL/6 mice were exposed to 0.1 µg/kg/day DES, 10 µg/kg/day BPA, 0.01, 0.1, or 1.0 µg/kg/day EE(2) or vehicle from Gestation Day 11 to Postnatal Day 12 via a mini-osmotic pump in the dam. Developmental xenoestrogen exposure altered femoral geometry and strength assessed in adulthood by µCT and torsional strength analysis, respectively. Low dose EE(2), DES or BPA increased adult femur length. Exposure to the highest dose of EE(2) did not alter femur length, resulting in a non-monotonic dose response. Exposure to EE(2) and DES, but not BPA, decreased tensile strength. The combined effect of increased femur length and decreased tensile strength resulted in a trend toward decreased torsional ultimate strength and energy to failure. Taken together, these results suggest that exposure to developmental exposure to environmentally-relevant levels of xenoestrogens may negatively impact bone length and strength in adulthood.
