Translate this page to:
Swedish
In JoVE (1)
Other Publications (1)
Automatic Translation
This translation into Swedish was automatically generated.
English Version | Other Languages
Articles by Katrin Beilharz in JoVE
JoVE Immunology and Infection
Levande cell imaging av Bacillus subtilis Och Streptococcus pneumoniae Med hjälp av automatiserade Time-lapse Mikroskopi
Detta protokoll är en steg-för-steg förfarande för att övervaka enskilda cellens beteende olika bakterier i tiden med hjälp av automatiserade fluorescens time-lapse mikroskopi. Dessutom ger vi riktlinjer för hur man kan analysera mikroskopi bilder.
Other articles by Katrin Beilharz on PubMed
Regulation of Peptidoglycan Synthesis by Outer-membrane Proteins
Growth of the mesh-like peptidoglycan (PG) sacculus located between the bacterial inner and outer membranes (OM) is tightly regulated to ensure cellular integrity, maintain cell shape, and orchestrate division. Cytoskeletal elements direct placement and activity of PG synthases from inside the cell, but precise spatiotemporal control over this process is poorly understood. We demonstrate that PG synthases are also controlled from outside of the sacculus. Two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional PG synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new PG to the sacculus. LpoB shows partial septal localization, and our data suggest that the LpoB-PBP1B complex contributes to OM constriction during cell division. LpoA/LpoB and their PBP-docking regions are restricted to γ-proteobacteria, providing models for niche-specific regulation of sacculus growth.
