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In JoVE (1)
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Articles by Keith Mewis in JoVE
JoVE General
A tela de elevada capacidade para Biomining Atividade da celulase de Bibliotecas metagenomic
Microbiology and Immunology, University of British Columbia - UBC
Este protocolo descreve um ecrã de alta taxa de transferência para a atividade celulolítica de uma biblioteca metagenomic expressa em Escherichia coli. A tela é solução baseada e altamente automatizado, e usa um pot-química em 384 microplacas bem com a leitura final como uma medida de absorbância.
Other articles by Keith Mewis on PubMed
The Art and Design of Functional Metagenomic Screens
This article summarizes general design principles for functional metagenomics. The focus is on Escherichia coli as an expression host, although alternative host-vector systems are discussed in relation to optimizing gene recovery in activity-based screens. Examples of DNA isolation and enrichment approaches, library construction and phenotypic read-out are described with special emphasis on the use of high throughput technologies for rapid isolation of environmental clones encoding phenotypic traits of interest.
