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In JoVE (1)
Other Publications (1)
Articles by Leo Ayuk in JoVE
The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva
Cynthia L. Bristow1, Mariya A. Babayeva1, Rozbeh Modarresi1, Carole P. McArthur2, Santosh Kumar3, Charles Awasom4, Leo Ayuk4, Annette Njinda5, Paul Achu5, Ronald Winston6
1Department of Medicine, Weill Cornell Medical College, 2Department of Oral Biology, University of Missouri-Kansas City-School of Dentistry, 3Department of Pharmacology and Toxicology, University of Missouri Kansas City- School of Pharmacy, 4Regional Hospital, Bamenda, NWP, Cameroon, 5Mezam Polyclinic HIV/AIDS Treatment Center, Cameroon, 6Institute for Human Genetics and Biochemistry
A CD4 enumeration method, the α-test, is described which uses whole saliva to provide rapid and accurate CD4 counts. The α-test costs pennies and eliminates the need for technical training, costly reagents such as monoclonal antibodies, instrumentation, refrigeration, transport of samples, as well as collection and handling of blood.
Other articles by Leo Ayuk on PubMed
Dose-reduced Conditioning Regimen Followed by Allogeneic Stem Cell Transplantation in Patients with Myelofibrosis with Myeloid Metaplasia
British Journal of Haematology. Dec, 2002 | Pubmed ID: 12437657
Three patients with myelofibrosis received allogeneic stem cell transplantation after a dose-reduced conditioning regimen of busulphan (8 mg/kg), fludarabine (180 mg/m2) and antithymocyte globulin (4 x 10 mg/kg). The median age at transplantation was 51 years (range 44-58). All patients engrafted with a leucocyte count > 1.0 x 10(9)/l after a median of 18 d (range 16-20). Grade II acute skin graft-versus-host disease (GvHD) occurred in one patient. One limited and one extensive chronic GvHD was observed. All patients achieved complete haematological remission. In one patient the fibrosis resolved completely 180 d post transplant. All patients are alive 126, 466 and 764 d after transplantation.