Articles by M. Idalia Cruz in JoVE

Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats

JoVE 2260 10/16/2010

Sonia de Assis¹, Anni Warri^1,2, M. Idalia Cruz¹, Leena Hilakivi-Clarke¹

¹Department of Oncology, Georgetown University, ²Institute of Biomedicine, University of Turku Medical Faculty

Our protocol describes how to dissect the rat abdominal mammary gland and how to prepare mammary gland whole mounts. It also describes how to analyze mammary gland morphology using three end-points (number of terminal end buds, epithelial elongation and differentiation) and to use these results to predict mammary cancer risk in rats which were exposed to dietary modifications in utero or during prepuberty.