Mark Helm The Pharmacy and Biochemistry Institute Johannes Gutenberg University Biography Publications Institution JoVE Articles Mark Helm has not added a biography. If you are Mark Helm and would like to personalize this page please email our Author Liaison for assistance. Publications Identification of Modifications in Microbial, Native TRNA That Suppress Immunostimulatory Activity The Journal of Experimental Medicine. Feb, 2012 | Pubmed ID: 22312113 Dye Selection for Live Cell Imaging of Intact SiRNA Biological Chemistry. Dec, 2011 | Pubmed ID: 22148896 RNA Nucleotide Methylation Wiley Interdisciplinary Reviews. RNA. Sep-Oct, 2011 | Pubmed ID: 21823225 Single-molecule FRET Reveals a Cooperative Effect of Two Methyl Group Modifications in the Folding of Human Mitochondrial TRNA(Lys) Chemistry & Biology. Jul, 2011 | Pubmed ID: 21802013 Regulatory RNAs and Beyond EMBO Reports. Aug, 2011 | Pubmed ID: 21760614 Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA Journal of Nucleic Acids. 2011 | Pubmed ID: 21716696 A Multifunctional Bioconjugate Module for Versatile Photoaffinity Labeling and Click Chemistry of RNA Nucleic Acids Research. Sep, 2011 | Pubmed ID: 21646334 Single-molecule FRET Studies of Counterion Effects on the Free Energy Landscape of Human Mitochondrial Lysine TRNA Biochemistry. Apr, 2011 | Pubmed ID: 21375355 A Post-labeling Approach for the Characterization and Quantification of RNA Modifications Based on Site-directed Cleavage by DNAzymes Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21370054 Measuring Single Small Molecule Binding Via Rupture Forces of a Split Aptamer Journal of the American Chemical Society. Feb, 2011 | Pubmed ID: 21287983 Expanding the Chemical Scope of RNA:methyltransferases to Site-specific Alkynylation of RNA for Click Labeling Nucleic Acids Research. Mar, 2011 | Pubmed ID: 21037259 RNA Methylation by Dnmt2 Protects Transfer RNAs Against Stress-induced Cleavage Genes & Development. Aug, 2010 | Pubmed ID: 20679393 TRNA Stabilization by Modified Nucleotides Biochemistry. Jun, 2010 | Pubmed ID: 20459084 Detection of RNA Modifications RNA Biology. Mar-Apr, 2010 | Pubmed ID: 20224293 A New Nuclear Function of the Entamoeba Histolytica Glycolytic Enzyme Enolase: the Metabolic Regulation of Cytosine-5 Methyltransferase 2 (Dnmt2) Activity PLoS Pathogens. Feb, 2010 | Pubmed ID: 20174608 Evidence-based Use of Second-generation Antipsychotics in a State Medicaid Pediatric Population, 2001-2005 Psychiatric Services (Washington, D.C.). Feb, 2010 | Pubmed ID: 20123816 Formation of a Stalled Early Intermediate of Pseudouridine Synthesis Monitored by Real-time FRET RNA (New York, N.Y.). Mar, 2010 | Pubmed ID: 20106954 FRET Imaging of Cells Transfected with SiRNA/liposome Complexes Methods in Molecular Biology (Clifton, N.J.). 2010 | Pubmed ID: 20013414 5-methylcytosine in RNA: Detection, Enzymatic Formation and Biological Functions Nucleic Acids Research. Mar, 2010 | Pubmed ID: 20007150 In Vivo Down-regulation of Mouse Brain Capillary P-glycoprotein: a Preliminary Investigation Neuroscience Letters. Oct, 2009 | Pubmed ID: 19679165 Good and Poor Adherence: Optimal Cut-point for Adherence Measures Using Administrative Claims Data Current Medical Research and Opinion. Sep, 2009 | Pubmed ID: 19635045 Effect of a Quaternary Pentamine on RNA Stabilization and Enzymatic Methylation Biological Chemistry. Sep, 2009 | Pubmed ID: 19558320 Prospective Validation of Eight Different Adherence Measures for Use with Administrative Claims Data Among Patients with Schizophrenia Value in Health : the Journal of the International Society for Pharmacoeconomics and Outcomes Research. Sep, 2009 | Pubmed ID: 19402852 Preparation of Small Amounts of Sterile SiRNA-liposomes with High Entrapping Efficiency by Dual Asymmetric Centrifugation (DAC) Journal of Controlled Release : Official Journal of the Controlled Release Society. Apr, 2009 | Pubmed ID: 19124051 An Empirical Basis for Standardizing Adherence Measures Derived from Administrative Claims Data Among Diabetic Patients Medical Care. Nov, 2008 | Pubmed ID: 18953222 RNA Intramolecular Dynamics by Single-molecule FRET Current Protocols in Nucleic Acid Chemistry / Edited by Serge L. Beaucage ... [et Al.]. Sep, 2008 | Pubmed ID: 18819081 Human DNMT2 Methylates TRNA(Asp) Molecules Using a DNA Methyltransferase-like Catalytic Mechanism RNA (New York, N.Y.). Aug, 2008 | Pubmed ID: 18567810 Sculpting an RNA Conformational Energy Landscape by a Methyl Group Modification--a Single-molecule FRET Study Angewandte Chemie (International Ed. in English). 2008 | Pubmed ID: 18449867 Use of DNAzymes for Site-specific Analysis of Ribonucleotide Modifications RNA (New York, N.Y.). Jan, 2008 | Pubmed ID: 17998290 Readout of Micromechanical Cantilever Sensor Arrays by Fabry-Perot Interferometry The Review of Scientific Instruments. Oct, 2007 | Pubmed ID: 17979440 A Methyl Group Controls Conformational Equilibrium in Human Mitochondrial TRNA(Lys) Journal of the American Chemical Society. Nov, 2007 | Pubmed ID: 17941640 Surveillance of SiRNA Integrity by FRET Imaging Nucleic Acids Research. 2007 | Pubmed ID: 17890733 Mg2+-dependent Folding of a Diels-Alderase Ribozyme Probed by Single-molecule FRET Analysis Nucleic Acids Research. 2007 | Pubmed ID: 17344321 Molecular Dysfunction Associated with the Human Mitochondrial 3302A>G Mutation in the MTTL1 (mt-tRNALeu(UUR)) Gene Nucleic Acids Research. 2006 | Pubmed ID: 17130166 Post-transcriptional Nucleotide Modification and Alternative Folding of RNA Nucleic Acids Research. 2006 | Pubmed ID: 16452298 Optimizing Splinted Ligation of Highly Structured Small RNAs RNA (New York, N.Y.). Dec, 2005 | Pubmed ID: 16251384 A New Mechanism for MtDNA Pathogenesis: Impairment of Post-transcriptional Maturation Leads to Severe Depletion of Mitochondrial TRNASer(UCN) Caused by T7512C and G7497A Point Mutations Nucleic Acids Research. 2005 | Pubmed ID: 16199753 Allosterically Activated Diels-Alder Catalysis by a Ribozyme Journal of the American Chemical Society. Aug, 2005 | Pubmed ID: 16045328 Aminoacylation Properties of Pathology-related Human Mitochondrial TRNA(Lys) Variants RNA (New York, N.Y.). May, 2004 | Pubmed ID: 15100439 Nuclear Control of Cloverleaf Structure of Human Mitochondrial TRNA(Lys) Journal of Molecular Biology. Mar, 2004 | Pubmed ID: 15019776 A Monomer-to-trimer Transition of the Human Mitochondrial Transcription Termination Factor (mTERF) is Associated with a Loss of in Vitro Activity The Journal of Biological Chemistry. Apr, 2004 | Pubmed ID: 14744862 RNA Sex Chemistry & Biology. Dec, 2003 | Pubmed ID: 14700622 Variability of Substrate Specificity of Serum Antibodies Obtained from Patients with Different Autoimmune and Viral Diseases in Reaction of TRNA Hydrolysis Russian Journal of Immunology : RJI : Official Journal of Russian Society of Immunology. Apr, 1999 | Pubmed ID: 12687113 In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme Ayala Tovy1, Benjamin Hofmann2, Mark Helm2, Serge Ankri1 1Faculty of Medicine, Rappaport Institute, Technion - Israel Institute of Technology, 2The Pharmacy and Biochemistry Institute, Johannes Gutenberg University JoVE 2390 Immunology and Infection
In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme Ayala Tovy1, Benjamin Hofmann2, Mark Helm2, Serge Ankri1 1Faculty of Medicine, Rappaport Institute, Technion - Israel Institute of Technology, 2The Pharmacy and Biochemistry Institute, Johannes Gutenberg University JoVE 2390 Immunology and Infection
