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In JoVE (1)
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Articles by Matthew Sulkin in JoVE
JoVE Bioengineering
מיפוי אופטי של פוטנציאלים פעולה ו הארעית סידן בלב מאוס
Department of Biomedical Engineering, Washington University in St. Louis
מאמר זה מפרט את הליך הנתיחה, הגדרת תפקיד, ואת תנאי הניסוי במהלך מיפוי אופטי פוטנציאל הטרנסממברני (VM) וסידן תאיים חולף (CAT) ב Langendorff מבודד בלב שלם perfused העכבר.
Other articles by Matthew Sulkin on PubMed
Selective Autoretroperfusion Preserves Myocardial Function During Coronary Artery Ligation in Swine
External pumps have been previously used to minimize edema and hemorrhage caused by coronary retroperfusion. The objective of this study was to use a pump-less approach (selective autoretroperfusion, SARP) to preserve myocardial function after acute coronary artery ligation.
Three Potential Mechanisms for Failure of HIFU Ablation in Cardiac Tissue
BACKGROUND: -High Intensity Focused Ultrasound (HIFU) has been introduced for treatment of cardiac arrhythmias, because it offers the ability to create rapid tissue modification in confined volumes without directly contacting the myocardium. In spite of the benefits of HIFU, a number of limitations have been reported, which hindered its clinical adoption. METHODS AND RESULTS: -In this study, we used a multimodal approach to evaluate thermal and non-thermal effects of HIFU in cardiac ablation. We designed a computer-controlled system capable of simultaneous fluorescence mapping and HIFU ablation. Using this system, linear lesions were created in isolated rabbit atria (n = 6) and point lesions were created in the ventricles of whole-heart (n = 6) preparations by applying HIFU at clinical doses (4-16W). Additionally, we evaluate the gap size in ablation lines necessary for conduction in atrial preparations (n = 4). The voltage sensitive dye di-4-ANEPPS was used to assess functional damage produced by HIFU. Optical coherence tomography and general histology were used to evaluate lesion extent. Conduction block was achieved in 1 (17%) of 6 atrial preparations with a single ablation line. Following 10 minutes of rest, 0 (0%) of 6 atrial preparations demonstrated sustained conduction block from a single ablation line. Tissue displacement of 1-3mm was observed during HIFU application due to acoustic radiation force along the lesion line. Additionally, excessive acoustic pressure and high temperature from HIFU generated cavitation causing macroscopic tissue damage. A minimum gap size of 1.5mm was found to conduct electrical activity. CONCLUSIONS: -This study identified three potential mechanisms responsible for the failure of HIFU ablation in cardiac tissues. Both acoustic radiation force and acoustic cavitation in conjunction with inconsistent thermal deposition can increase the risk of lesion discontinuity and result in gap sizes that promote ablation failure.
