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Articles by Meera Rao in JoVE
JoVE General
Kararlı İzotopik Profil geliştirilmesi ve Yetişkin Sahne Aracı Metabolik Akı Caenorhabditis elegans
1Department of Pediatrics, The Children's Hospital of Philadelphia, 2Department of Pediatrics, University of Pennsylvania
Kararlı izotop profil aracı metabolik akı gaz kromatografisi kütle spektrometrik analizi, nematod açıklanan
Other articles by Meera Rao on PubMed
Mitochondrial Respiratory Chain Dysfunction Variably Increases Oxidant Stress in Caenorhabditis Elegans
Mitochondrial dysfunction and associated oxidant stress have been linked with numerous complex diseases and aging largely by in vitro determination of mitochondria oxidant production and scavenging. We applied targeted in vivo fluorescence analyses of mitochondria-dense pharyngeal tissue in Caenorhabditis elegans to better understand relative mitochondrial effects, particularly on matrix oxidant burden, of respiratory chain complex, MnSOD, and insulin receptor mutants displaying variable longevity. The data demonstrate significantly elevated in vivo matrix oxidant burden in the short-lived complex I mutant, gas-1(fc21), which was associated with limited superoxide scavenging capacity despite robust MnSOD induction, as well as decreased mitochondria content and membrane potential. Significantly increased MnSOD activity was associated with in vivo matrix oxidant levels similar to wild-type in the long-lived respiratory chain complex III mutant, isp-1(qm150). Yet, despite greater superoxide scavenging capacity in the complex III mutant than in the significantly longer-lived insulin receptor mutant, daf-2(e1368), only the former showed modest oxidative stress sensitivity. Furthermore, increased longevity was seen in MnSOD knockout mutants (sod-2(ok1030) and sod-2(gk257)) that had decreased MnSOD scavenging capacity and increased in vivo matrix oxidant burden. Thus, factors beside oxidant stress must underlie RC mutant longevity in C. elegans. This work highlights the utility of the C. elegans model as a tractable means to non-invasively monitor multi-dimensional in vivo consequences of primary mitochondrial dysfunction.
Propargyl Alcohols As β-oxocarbenoid Precursors for the Ruthenium-catalyzed Cyclopropanation of Unactivated Olefins by Redox Isomerization
An atom-economical method for the direct synthesis of [3.1.0]- and [4.1.0]-bicyclic frameworks via Ru-catalyzed redox bicycloisomerization of enynols is reported. The presented results highlight the unique reactivity profile of propargyl alcohols, which function as β-oxocarbene precursors, in the presence of a ruthenium(II) complex. Furthermore, a rare case of a formal vinylic C-H insertion reaction is described.
The Get5 Carboxyl Terminal Domain is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex
Tail-anchored (TA) transmembrane proteins are targeted to membranes post- translationally. The proteins Get4 and Get5 form an obligate complex that catalyzes the transfer of TA proteins destined to the endoplasmic reticulum (ER) from Sgt2 to the cytosolic targeting factor Get3. Get5 forms a homodimer mediated by its carboxyl domain. We show here that a conserved motif exists within the carboxyl domain. A high-resolution crystal structure and solution NMR structures of this motif reveal a novel and stable helical dimerization domain. We additionally determined a solution NMR structure of a divergent fungal homolog and comparison of these structures allows annotation of specific stabilizing interactions. Using solution X-ray scattering and the structures of all folded domains, we present a model of the full length Get4/Get5 complex.
