Articles by Miriam Votteler in JoVE

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

JoVE 3977 5/29/2012

Miriam Votteler^1,2, Daniel A. Carvajal Berrio², Marieke Pudlas^2,3, Heike Walles^2,4, Katja Schenke-Layland^1,2

¹Department of Thoracic and Cardiovascular Surgery and Inter-University Centre for Medical Technology Stuttgart-Tübingen (IZST), Eberhard Karls University, Tübingen, ²Department of Cell and Tissue Engineering, Fraunhofer Institute of Interfacial Engineering and Biotechnology (IGB) Stuttgart, Germany, ³Department for Medical Interfacial Engineering (IGVT), University of Stuttgart, Germany, ⁴Institute of Tissue Engineering and Regenerative Medicine, Julius-Maximillians University, Würzburg, Germany

Raman spectroscopy is a suitable technique for the non-contact, label-free analysis of living cells, tissue-engineered constructs and native tissues. Source-specific spectral fingerprints can be generated and analyzed using multivariate analysis.