Mitchel J. Doktycz Biosciences Division Oak Ridge National Laboratory Biography Publications Institution JoVE Articles Mitchel J. Doktycz has not added a biography. If you are Mitchel J. Doktycz and would like to personalize this page please email our Author Liaison for assistance. Publications Advances and Perspectives in Discovery and Functional Analysis of Small Secreted Proteins in Plants Horticulture Research. Jun, 2021 | Pubmed ID: 34059650 Formation, Characterization and Modeling of Emergent Synthetic Microbial Communities Computational and Structural Biotechnology Journal. 2021 | Pubmed ID: 33995895 A Lysate Proteome Engineering Strategy for Enhancing Cell-free Metabolite Production Metabolic Engineering Communications. Jun, 2021 | Pubmed ID: 33552897 Machine Learning-based Prediction of Enzyme Substrate Scope: Application to Bacterial Nitrilases Proteins. 03, 2021 | Pubmed ID: 33118210 Targeted Growth Medium Dropouts Promote Aromatic Compound Synthesis in Crude Cell-Free Systems ACS Synthetic Biology. 11, 2020 | Pubmed ID: 33044063 A Carotenoid-deficient Mutant of the Plant-associated Microbe Pantoea Sp. YR343 Displays an Altered Membrane Proteome Scientific Reports. 09, 2020 | Pubmed ID: 32917935 Computationally Guided Discovery and Experimental Validation of Indole-3-acetic Acid Synthesis Pathways ACS Chemical Biology. 12, 2019 | Pubmed ID: 31693336 Microfluidics and Metabolomics Reveal Symbiotic Bacterial-Fungal Interactions Between and Include Metabolite Exchange Frontiers in Microbiology. 2019 | Pubmed ID: 31632357 Label-free Time- and Space-resolved Exometabolite Sampling of Growing Plant Roots Through Nanoporous Interfaces Scientific Reports. 07, 2019 | Pubmed ID: 31312009 Pore-scale Hydrodynamics Influence the Spatial Evolution of Bacterial Biofilms in a Microfluidic Porous Network PloS One. 2019 | Pubmed ID: 31246972 Increasing Access to Microfluidics for Studying Fungi and Other Branched Biological Structures Fungal Biology and Biotechnology. 2019 | Pubmed ID: 31198578 Loss of Carotenoids from Membranes of Pantoea Sp. YR343 Results in Altered Lipid Composition and Changes in Membrane Biophysical Properties Biochimica Et Biophysica Acta. Biomembranes. 07, 2019 | Pubmed ID: 31095944 Itaconic Acid Degradation in : the Role of Unexpected Bioconversion Pathways Fungal Biology and Biotechnology. 2019 | Pubmed ID: 30622724 Elucidating the Potential of Crude Cell Extracts for Producing Pyruvate from Glucose Synthetic Biology (Oxford, England). 2018 | Pubmed ID: 32995514 Exploration of the Biosynthetic Potential of the Microbiome MSystems. Sep-Oct, 2018 | Pubmed ID: 30320216 Elucidating Duramycin's Bacterial Selectivity and Mode of Action on the Bacterial Cell Envelope Frontiers in Microbiology. 2018 | Pubmed ID: 29491859 Nano-Enabled Approaches to Chemical Imaging in Biosystems Annual Review of Analytical Chemistry (Palo Alto, Calif.). 06, 2018 | Pubmed ID: 29490189 Abiotic Stresses Shift Belowground -Associated Bacteria Toward a Core Stress Microbiome MSystems. Jan-Feb, 2018 | Pubmed ID: 29404422 Automated Interpretation and Extraction of Topographic Information from Time of Flight Secondary Ion Mass Spectrometry Data Scientific Reports. 12, 2017 | Pubmed ID: 29213083 β-(1,3)-Glucan Unmasking in Some Candida Albicans Mutants Correlates with Increases in Cell Wall Surface Roughness and Decreases in Cell Wall Elasticity Infection and Immunity. Jan, 2017 | Pubmed ID: 27849179 Enrichment of Root Endophytic Bacteria from Populus Deltoides and Single-Cell-Genomics Analysis Applied and Environmental Microbiology. Sep, 2016 | Pubmed ID: 27422831 While-you-wait Proteins? Producing Biomolecules at the Point of Need Expert Review of Proteomics. Aug, 2016 | Pubmed ID: 27402489 Two Poplar-Associated Bacterial Isolates Induce Additive Favorable Responses in a Constructed Plant-Microbiome System Frontiers in Plant Science. 2016 | Pubmed ID: 27200001 Stochastic Assembly of Bacteria in Microwell Arrays Reveals the Importance of Confinement in Community Development PloS One. 2016 | Pubmed ID: 27152511 A Carotenoid-Deficient Mutant in Pantoea Sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus Deltoides, Is Defective in Root Colonization Frontiers in Microbiology. 2016 | Pubmed ID: 27148182 Toward Microfluidic Reactors for Cell-Free Protein Synthesis at the Point-of-Care Small (Weinheim an Der Bergstrasse, Germany). Feb, 2016 | Pubmed ID: 26690885 Microstencils to Generate Defined, Multi-species Patterns of Bacteria Biomicrofluidics. Nov, 2015 | Pubmed ID: 26594264 Fabrication of Nanoporous Membranes for Tuning Microbial Interactions and Biochemical Reactions Journal of Vacuum Science and Technology. B, Nanotechnology & Microelectronics : Materials, Processing, Measurement, & Phenomena : JVST B. Nov, 2015 | Pubmed ID: 26543684 Metabolic Functions of Pseudomonas Fluorescens Strains from Populus Deltoides Depend on Rhizosphere or Endosphere Isolation Compartment Frontiers in Microbiology. 2015 | Pubmed ID: 26528266 New Surface Radiolabeling Schemes of Super Paramagnetic Iron Oxide Nanoparticles (SPIONs) for Biodistribution Studies Nanoscale. Apr, 2015 | Pubmed ID: 25790032 Microstructured Block Copolymer Surfaces for Control of Microbe Adhesion and Aggregation Biosensors. Mar, 2014 | Pubmed ID: 25587410 Evaluation and Validation of De Novo and Hybrid Assembly Techniques to Derive High-quality Genome Sequences Bioinformatics (Oxford, England). Oct, 2014 | Pubmed ID: 24930142 Multi-input Regulation and Logic with T7 Promoters in Cells and Cell-free Systems PloS One. 2013 | Pubmed ID: 24194933 Enteroaggregative Escherichia Coli: Surface Protein Dispersin Increases Bacterial Uptake of Ciprofloxacin International Journal of Antimicrobial Agents. Nov, 2013 | Pubmed ID: 24011504 Layer-by-layer Templated Assembly of Silica at the Nanoscale Langmuir : the ACS Journal of Surfaces and Colloids. Feb, 2013 | Pubmed ID: 23360298 Relating Nanomaterial Properties and Microbial Toxicity Nanoscale. Jan, 2013 | Pubmed ID: 23203029 Monodisperse Alginate Microgel Formation in a Three-dimensional Microfluidic Droplet Generator Biomicrofluidics. 2012 | Pubmed ID: 24198865 Twenty-one Genome Sequences from Pseudomonas Species and 19 Genome Sequences from Diverse Bacteria Isolated from the Rhizosphere and Endosphere of Populus Deltoides Journal of Bacteriology. Nov, 2012 | Pubmed ID: 23045501 Draft Genome Sequence of Rhizobium Sp. Strain PDO1-076, a Bacterium Isolated from Populus Deltoides Journal of Bacteriology. May, 2012 | Pubmed ID: 22493196 Pseudomonas Fluorescens Induces Strain-dependent and Strain-independent Host Plant Responses in Defense Networks, Primary Metabolism, Photosynthesis, and Fitness Molecular Plant-microbe Interactions : MPMI. Jun, 2012 | Pubmed ID: 22375709 Cytotoxicity Induced by Engineered Silver Nanocrystallites is Dependent on Surface Coatings and Cell Types Langmuir : the ACS Journal of Surfaces and Colloids. Feb, 2012 | Pubmed ID: 22216981 Enzyme Reactions in Nanoporous, Picoliter Volume Containers Analytical Chemistry. Jan, 2012 | Pubmed ID: 22148720 Expression Optimization and Synthetic Gene Networks in Cell-free Systems Nucleic Acids Research. Dec, 2011 | Pubmed ID: 22180537 The Chemotaxis-like Che1 Pathway Has an Indirect Role in Adhesive Cell Properties of Azospirillum Brasilense FEMS Microbiology Letters. Oct, 2011 | Pubmed ID: 22092709 Monodispersed Biocompatible Silver Sulfide Nanoparticles: Facile Extracellular Biosynthesis Using the γ-proteobacterium, Shewanella Oneidensis Acta Biomaterialia. Dec, 2011 | Pubmed ID: 21798382 Distinct Microbial Communities Within the Endosphere and Rhizosphere of Populus Deltoides Roots Across Contrasting Soil Types Applied and Environmental Microbiology. Sep, 2011 | Pubmed ID: 21764952 Model for Biological Communication in a Nanofabricated Cell-mimic Driven by Stochastic Resonance Nano Communication Networks. Mar, 2011 | Pubmed ID: 21731597 Effects of Sub-minimum Inhibitory Concentrations of Ciprofloxacin on Enteroaggregative Escherichia Coli and the Role of the Surface Protein Dispersin International Journal of Antimicrobial Agents. Jul, 2011 | Pubmed ID: 21570813 Biofabrication of Discrete Spherical Gold Nanoparticles Using the Metal-reducing Bacterium Shewanella Oneidensis Acta Biomaterialia. May, 2011 | Pubmed ID: 21241833 Characterization of Cell Surface and Extracellular Matrix Remodeling of Azospirillum Brasilense Chemotaxis-like 1 Signal Transduction Pathway Mutants by Atomic Force Microscopy FEMS Microbiology Letters. Jan, 2011 | Pubmed ID: 21105907 Nanostructured Silicon Membranes for Control of Molecular Transport Journal of Vacuum Science and Technology. B, Nanotechnology & Microelectronics : Materials, Processing, Measurement, & Phenomena : JVST B. Nov, 2010 | Pubmed ID: 24932436 Effects of Engineered Cerium Oxide Nanoparticles on Bacterial Growth and Viability Applied and Environmental Microbiology. Dec, 2010 | Pubmed ID: 20952651 Atomic Force Microscopy of Biological Samples Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology. Nov-Dec, 2010 | Pubmed ID: 20672388 Surface Charge- and Space-dependent Transport of Proteins in Crowded Environments of Nanotailored Posts ACS Nano. Jun, 2010 | Pubmed ID: 20515056 Silver Nanocrystallites: Biofabrication Using Shewanella Oneidensis, and an Evaluation of Their Comparative Toxicity on Gram-negative and Gram-positive Bacteria Environmental Science & Technology. Jul, 2010 | Pubmed ID: 20509652 Development and Fabrication of Nanoporous Silicon-based Bioreactors Within a Microfluidic Chip Lab on a Chip. May, 2010 | Pubmed ID: 20390137 Cell Free Translation in Engineered Picoliter Volume Containers Annual ORNL Biomedical Science and Engineering Center Conference ORNL Biomedical Science and Engineering Center Conference. Jun, 2009 | Pubmed ID: 21278819 An in Vivo Imaging-based Assay for Detecting Protein Interactions over a Wide Range of Binding Affinities Analytical Biochemistry. Dec, 2009 | Pubmed ID: 19698693 Effects of Ultramicroelectrode Dimensions on the Electropolymerization of Polypyrrole Journal of Applied Physics. Jun, 2009 | Pubmed ID: 19657404 Positional Control of Catalyst Nanoparticles for the Synthesis of High Density Carbon Nanofiber Arrays Carbon. 2008 | Pubmed ID: 19448842 Actuatable Membranes Based on Polypyrrole-coated Vertically Aligned Carbon Nanofibers ACS Nano. Feb, 2008 | Pubmed ID: 19206624 A General System for Studying Protein-protein Interactions in Gram-negative Bacteria Journal of Proteome Research. Aug, 2008 | Pubmed ID: 18590317 Controlling the Dimensions of Carbon Nanofiber Structures Through the Electropolymerization of Pyrrole Synthetic Metals. Apr, 2007 | Pubmed ID: 18431459 Nano-enabled Synthetic Biology Molecular Systems Biology. 2007 | Pubmed ID: 17625513 The Near-naked Hairless (Hr(N)) Mutation Disrupts Hair Formation but is Not Due to a Mutation in the Hairless Coding Region The Journal of Investigative Dermatology. Jul, 2007 | Pubmed ID: 17330134 Evaluation of a Surface-sampling Probe Electrospray Mass Spectrometry System for the Analysis of Surface-deposited and Affinity-captured Proteins Rapid Communications in Mass Spectrometry : RCM. 2006 | Pubmed ID: 16521171 Optimized Beadmilling of Tissues for High-throughput RNA Production and Microarray-based Analyses Analytical Biochemistry. Sep, 2004 | Pubmed ID: 15301954 AFM Imaging of Bacteria in Liquid Media Immobilized on Gelatin Coated Mica Surfaces Ultramicroscopy. Oct-Nov, 2003 | Pubmed ID: 12801673 Fingerprinting of Prokaryotic 16S RRNA Genes Using Oligodeoxyribonucleotide Microarrays and Virtual Hybridization Nucleic Acids Research. Jan, 2003 | Pubmed ID: 12527788 Genomes to Life "Center for Molecular and Cellular Systems": a Research Program for Identification and Characterization of Protein Complexes Omics : a Journal of Integrative Biology. 2002 | Pubmed ID: 12630408 Comparative Analyses of the Secondary Structures of Synthetic and Intracellular Yeast MFA2 MRNAs Proceedings of the National Academy of Sciences of the United States of America. Dec, 1998 | Pubmed ID: 9843938 Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems Jaime Lorenzo N. Dinglasan1,3, David T. Reeves2,3, Robert L. Hettich3, Mitchel J. Doktycz3 1Graduate School of Genome Science & Technology, University of Tennessee Knoxville, 2Bredesen Center for Interdisciplinary Research, University of Tennessee Knoxville, 3Biosciences Division, Oak Ridge National Laboratory JoVE 62852 Bioengineering Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform Jayde A. Aufrecht1,2, Jennifer M. Ryan3, Sahar Hasim4, David P. Allison2,3, Andreas Nebenführ3, Mitchel J. Doktycz1,2, Scott T. Retterer1,2 1Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, 2Bioscience Division and Center for Nanophase Materials Sciences, Oak Ridge national Laboratory, 3Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 4Department of Microbiology, University of Tennessee JoVE 55971 Bioengineering
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems Jaime Lorenzo N. Dinglasan1,3, David T. Reeves2,3, Robert L. Hettich3, Mitchel J. Doktycz3 1Graduate School of Genome Science & Technology, University of Tennessee Knoxville, 2Bredesen Center for Interdisciplinary Research, University of Tennessee Knoxville, 3Biosciences Division, Oak Ridge National Laboratory JoVE 62852 Bioengineering
Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform Jayde A. Aufrecht1,2, Jennifer M. Ryan3, Sahar Hasim4, David P. Allison2,3, Andreas Nebenführ3, Mitchel J. Doktycz1,2, Scott T. Retterer1,2 1Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, 2Bioscience Division and Center for Nanophase Materials Sciences, Oak Ridge national Laboratory, 3Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 4Department of Microbiology, University of Tennessee JoVE 55971 Bioengineering
