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In JoVE (1)
Other Publications (1)
Articles by Mohammed M. Islam in JoVE
In Ovo Electroporation in Embryonic Chick Retina
Mohammed M. Islam1, Sung Tae Doh2, Li Cai1,2
1Department of Pharmacology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 2Department of Biomedical Engineering, Rutgers University
The overall goal of this video is to show how to perform targeted retinal injection and in ovo electroporation of DNA/RNA constructs into the chick embryonic retina at the Hamburger and Hamilton stage 22-23, which is about embryonic day 4 (E4). This technique is very useful to study gene expression, gene regulation, and morphological change in developing chick retina.
Other articles by Mohammed M. Islam on PubMed
The Journal of Nutrition. Nov, 2003 | Pubmed ID: 14672305
Fish play an important role in the Bangladeshi diet, constituting the main and often irreplaceable animal source food in poor rural households. Fish consumption is dominated by wild small (length <25 cm) indigenous fish species (SIS). The vitamin A content in SIS varies, from <100 microg of retinol equivalents (RE)/100 g raw edible parts, to >2,500 microg RE/100 g raw edible parts in mola (Amblypharyngodon mola). The study addressed the dietary contribution of fish to vitamin A, calcium and iron intakes and the potential of integrating SIS, including mola, into existing carp polyculture ponds. Fish consumption (wild and cultured fish) was surveyed by 5-d recall interviews in 84 poor rural households in Kishoreganj district in 1997-1998. Fifty-nine of the households cultured carp and SIS in small (mean size 400 m2) domestic ponds. Total household fish consumption was unaffected by the domestic aquaculture production. SIS from wild sources contributed 84% of the total fish consumption. In the peak season (October), SIS contributed 40% (median 23%) of the recommended vitamin A intake at the household level (n = 84). Thirty-four households cultured mola along with carp. Cultured mola used for household consumption contributed 20% (median 18%) of the recommended intake of vitamin A at the household level. Wild SIS is an important source of vitamin A and calcium in Bangladesh. Mola can be integrated in existing carp culture without negative effects and can contribute to increased vitamin A intake in rural households.