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In JoVE (1)
Other Publications (6)
- Angewandte Chemie (International Ed. in English)
- Angewandte Chemie (International Ed. in English)
- Angewandte Chemie (International Ed. in English)
- Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine
- Journal of the American Chemical Society
- Chemical Communications (Cambridge, England)
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Articles by Nico A.J.M. Sommerdijk in JoVE
JoVE Applied Physics
Su Supramoleküler Polimerlerin Boyut, Şekil ve Stabilite Kontrolü
1Organic Chemistry Institute and CeNTech, Westfälische Wilhelms-Universität Münster, 2Laboratory of Macromolecular and Organic Chemistry, Institute for Complex Molecular Systems, Eindhoven University of Technology, 3Laboratory of Materials and Interface Chemistry and Soft Matter Research Unit, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology
Bu deneyin amacı, belirlemek ve su içerisinde kendi kendine monte diskotik amfifiler büyüklüğü, şekli ve stabilite kontrol etmektir. Su bazlı supramoleküler polimerler için bu kontrol seviyesi çok zordur. Biz itici ve çekici etkileşimleri hem kullanarak bir strateji uygulamak. Bu sistem karakterize etmek için uygulanan deney yöntemleri genel olarak uygulanabilir.
Other articles by Nico A.J.M. Sommerdijk on PubMed
Cover Picture
The cover picture shows the process of building functional amphiphilic protein-polymer hybrids in a modular fashion. Monolayers of biotinylated polystyrene (purple) bind the protein streptavidin (blue), resulting in the formation of giant amphiphiles. The remaining free binding sites are subsequently used to associate biotinylated biomacromolecules (yellow), such as the iron-storage protein ferritin. The use of covalent conjugates of streptavidin and horseradish peroxidase leads to the formation of reactive surfaces, which are capable of catalyzing organic reactions. Further details on these giant amphiphiles are reported by Nolte and co-workers on p. 4732 ff.
Protein-Polymer Hybrid Amphiphiles This Research Was Supported by the Netherlands Foundation for Chemical Research (CW-NWO), the EC TMR Sisitomas and ESF Smarton Programs, and the Ministerio De Educación Y Cultura (Spain). The Authors Thank H. P. M. Geurts, P. J. H. M. Adams, and J. L. J. Van Dongen for Experimental Assistance
Kinetics of Avidin-induced Clearance of Biotinylated Bimodal Liposomes for Improved MR Molecular Imaging
Dual labeled liposomes, carrying both paramagnetic and fluorescent lipids, were recently proposed as potent contrast agents for MR molecular imaging. These nanoparticles are coated with poly(ethylene glycol) (PEG) to increase their blood circulation half-life, which should allow extensive accumulation at the targeted site. To eliminate nonspecific blood pool signal from the MR images, the circulating liposomes should ideally be cleared from the circulation when sufficient target-specific contrast enhancement is obtained. To that aim, we designed an avidin chase that allowed controlled and rapid clearance of paramagnetic biotinylated liposomes from the blood circulation in C57BL/6 mice. Avidin-induced alterations in blood clearance kinetics and tissue distribution were studied quantitatively by determination of the Gd content in blood and tissue samples ex vivo. Intrinsic liposomal blood clearance showed bi-exponential behavior with half-lives t(1/2alpha) = 2.1 +/- 1.1 and t(1/2beta) = 15.1 +/- 5.4 hours, respectively. In contrast, the contrast agent was cleared from the blood by the avidin infusion to <1% of the initial dose within 4 hours. Avidin-induced liposomal blood clearance was also demonstrated in vivo by dynamic T(1)-weighted MRI. The ability to rapidly clear circulating contrast agents opens up exciting possibilities to study targeting kinetics, to increase the specificity of molecular MRI and to optimize nanoparticulate contrast agent formulations.
Well-defined, Multifunctional Nanostructures of a Paramagnetic Lipid and a Lipopeptide for Macrophage Imaging
In the field of nanomedicine there is a great demand for technologies that allow the creation of self-assembled structures of which the size and morphology can be accurately controlled. In the current study, we report a nanoparticle platform that is composed of a paramagnetic lipid and a fluorescently labeled lipopeptide. By judiciously controlling the ratio of the aforementioned amphiphilic molecules, a variety of well-defined nanosized supramolecular structures with different sizes and morphologies could be created. The hydrodynamic radii of the different structures were determined by dynamic light scattering. Cryo-TEM revealed the aggregate morphology to vary from small micellar structures to plate-like and even full grown ribbons of which the aspect ratios varied from a diameter of 5-8 nm to structures with a width of up to 25 nm and infinite length. Interestingly, nuclear magnetic resonance dispersion profiling revealed excellent properties for MRI and also showed that the relaxivity of the structures was tunable and morphology dependent. Finally, macrophage cells were treated with two selected nanoparticles and were shown to be avidly taken up. In conclusion we demonstrate a methodology to create structures that (1) are paramagnetic to enable their detection with MRI, (2) exhibit fluorescent properties, (3) can be tuned to defined sizes and shapes, and (4) are efficiently taken up by macrophage cells in vitro.
The Binding of CNA35 Contrast Agents to Collagen Fibrils
CryoTEM demonstrates that a CNA35-bearing liposomal MRI contrast agent selectively binds to poorly assembled collagen type I as opposed to well-assembled collagen fibrils, whereas monomeric CNA35 binds to all forms of collagen. It is shown that upon conjugation to liposomes and micelles CNA35 loses its ability to dissociate ordered collagen fibrils and thereby to create its own binding sites.
