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In JoVE (1)
Other Publications (1)
Articles by Rachel Formosa in JoVE
DNA-based Fish Species Identification Protocol
Rachel Formosa, Harini Ravi, Scott Happe, Danielle Huffman, Natalia Novoradovskaya, Robert Kincaid, Steve Garrett
This publication describes how to use the Agilent Fish Species Identification System to identify the species of a fish by extracting DNA and performing PCR and RFLP analysis.
Other articles by Rachel Formosa on PubMed
Methods (San Diego, Calif.). Apr, 2010 | Pubmed ID: 20215015
The next-generation DNA sequencing workflows require an accurate quantification of the DNA molecules to be sequenced which assures optimal performance of the instrument. Here, we demonstrate the use of qPCR for quantification of DNA libraries used in next-generation sequencing. In addition, we find that qPCR quantification may allow improvements to current NGS workflows, including reducing the amount of library DNA required, increasing the accuracy in quantifying amplifiable DNA, and avoiding amplification bias by reducing or eliminating the need to amplify DNA before sequencing.