Articles by Robert Greenberg in JoVE

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

JoVE 3905 6/05/2012

Karen L. Joyce¹, Will Morgan², Robert Greenberg², Meera G. Nair¹

¹Institute of Immunology, Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, ²Pathobiology, School of Veterinary Medicine, University of Pennsylvania

Schistosoma mansoni eggs are potent stimulators of the T helper type 2 (Th2) immune response, characteristic of parasite infection, asthma and allergic inflammation. This protocol utilizes S. mansoni egg injection to generate a CD4 Th2 cytokine-induced inflammatory response in the lung, characterized by lung granuloma formation around the egg, eosinophilia and macrophage alternative activation.