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Articles by Ronald K. Siu in JoVE
JoVE Bioengineering
Uso de Células-tronco humanas perivasculares para Regeneração Óssea
1Dental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, UCLA, 2UCLA and Orthopaedic Hospital, Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA, 3Department of Bioengineering, UCLA, 4Center for Cardiovascular Science, University of Edinburgh
As células-tronco perivascular (PSC) é um romance classe de células-tronco para a regeneração do tecido ósseo semelhante a células-tronco mesenquimais (MSCs). PSCs pode ser isolado por FACS (classificação de células activadas por fluorescência) a partir de tecido adiposo adquiridos durante procedimentos padrão lipoaspiração, então combinada com um andaime osteoindutora para alcançar a formação de osso
Other articles by Ronald K. Siu on PubMed
Calvarial Cleidocraniodysplasia-Like Defects With ENU-Induced Nell-1 Deficiency
ABSTRACT: Nell-1, first identified by its overexpression in synostotic cranial sutures, is a novel osteoinductive growth and differentiation factor. To further define Nell-1's role in craniofacial patterning, we characterized defects of the ENU-induced Nell-1-deficient (END) mice, focusing on both intramembranous and endochondral cranial bones. Results showed that calvarial bones of neonatal END mice were reduced in thickness and density, with a phenotype resembling calvarial cleidocraniodysplasia. In addition, a global reduction in osteoblast markers was observed, including reductions in Runx2, alkaline phosphatase, and osteocalcin. Remarkably, detailed analysis of endochondral bones showed dysplasia as well. The chondrocranium in the END mouse showed enrichment for early, proliferating Sox9 chondrocytes, whereas in contrast markers of chondrocytes maturation were reduced. These data suggest that Nell-1 is an important growth factor for regulation of osteochondral differentiation, by regulating both Runx2 and Sox9 expression within the calvarium. In summary, Nell-1 is required for normal craniofacial membranous and endochondral skeletal development.
