Sébastien MAILFERT ImagImm: Photonic microscopy facility Centre d'Immunologie de Marseille Luminy Biography Publications Institution JoVE Articles Sébastien MAILFERT After a PhD in Eletronics obtained in 2006 on a multidisciplinary subject dealing with Electronics and Biology (Scanning Near-field Optical Microscopy and Atomic Force Microscopy applied on bacteria), I joined the Centre d'Immunologie de Marseille Luminy (CIML).Since 2007, I develop new microscopes and analysis methodologies applied to immunology, cellular membrane organization, etc.I have an expertise in multiple optical microscopy techniques such as for example spot variation Fluorescence Correlation Spectroscopy (svFCS), Single Molecule Localization Microscopy (SMLM), spectral confocal imaging, etc. Publications Author Correction: Phosphoinositides Regulate the TCR/CD3 Complex Membrane Dynamics and Activation Scientific Reports. Jul, 2020 | Pubmed ID: 32704037 Microtubule Plus-end Dynamics Link Wound Repair to the Innate Immune Response ELife. Jan, 2020 | Pubmed ID: 31995031 Phosphoinositides Regulate the TCR/CD3 Complex Membrane Dynamics and Activation Scientific Reports. 03, 2018 | Pubmed ID: 29563576 A Straightforward STED-background Corrected Fitting Model for Unbiased STED-FCS Analyses Methods (San Diego, Calif.). 05, 2018 | Pubmed ID: 29454082 Detection of Long-range Electrostatic Interactions Between Charged Molecules by Means of Fluorescence Correlation Spectroscopy Physical Review. E. Aug, 2017 | Pubmed ID: 28950524 Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells Sebastien Mailfert*1, Karolina Wojtowicz*2, Sophie Brustlein*1, Ewa Blaszczak3, Nicolas Bertaux4, Marcin Łukaszewicz2, Didier Marguet1, Tomasz Trombik2 1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ JoVE 61823 Biochemistry
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells Sebastien Mailfert*1, Karolina Wojtowicz*2, Sophie Brustlein*1, Ewa Blaszczak3, Nicolas Bertaux4, Marcin Łukaszewicz2, Didier Marguet1, Tomasz Trombik2 1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ JoVE 61823 Biochemistry
