JoVE Immunology and Infection
Emily D. Cisney, Stefan Fernandez, Shannan I. Hall, Gale A. Krietz, Robert G. Ulrich
U.S. Army Medical Research Institute of Infectious Diseases
Methods to examine contributions of the nasopharyngeal-associated lymphoreticular tissues (NALT) to nasal and systemic immune responses of mice to intranasal vaccines are described. We demonstrate a surgical procedure to establish a NALT-dependent mouse model and ex vivo cultures of extracted NALT.